دورية أكاديمية
Cytochrome P450 Herbicide Metabolism as the Main Mechanism of Cross-Resistance to ACCase- and ALS-Inhibitors in Lolium spp. Populations From Argentina: A Molecular Approach in Characterization and Detection
| العنوان: | Cytochrome P450 Herbicide Metabolism as the Main Mechanism of Cross-Resistance to ACCase- and ALS-Inhibitors in Lolium spp. Populations From Argentina: A Molecular Approach in Characterization and Detection |
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| المؤلفون: | Yanniccari, Marcos Ezequiel, Gigón, Ramón, Larsen, Adelina Olga |
| بيانات النشر: | Frontiers Media |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | MARKER-TRAIT ASSOCIATIONS, MULTIPLE-RESISTANCE, NON-TARGET SITE RESISTANCE (NTSR), RYEGRASS, TARGET-SITE RESISTANCE (TSR), https://purl.org/becyt/ford/4.1Test, https://purl.org/becyt/ford/4Test |
| الوصف: | Knowledge about the mechanisms of herbicide resistance provide valuable insights into evolving weed populations in response to selection pressure and should be used as a basis for designing management strategies for herbicide-resistant weeds. The selection pressure associated with reactive management against glyphosate-resistant Lolium spp. populations would have favored the herbicide resistance to ACCase- and ALS-inhibitors. This work was aimed to determine the sensitivity of 80 Argentinean Lolium spp. populations to ALS- and ACCase-inhibitor herbicides for use in wheat or barley and to study the mechanisms of resistance involved. Sensitivity to pinoxaden and iodosulfuron-mesosulfuron were positively correlated (r = 0.84), even though both affect different target sites. Inhibitors of cytochrome P450 monooxygenases (P450s) increased the sensitivity to pinoxaden and iodosulfuron-mesosulfuron in 94% of herbicide-resistant populations and target-site ACCase resistance mutations were detected only in two cases. Polymorphic variants were obtained with a pair primer designed on P450 sequences, cluster analysis discriminated around 80% of susceptible and P450-metabolic resistant plants sampled from a single population or different populations. Five markers corresponding to herbicide sensitivity were identified to be significantly associated with phenotypic variance in plants. Resistance to ALS- and ACCase-inhibitor herbicides were closely related, challenging the rotation of herbicides of both sites of action as a practice against resistance. In that sense, the use of pinoxaden and iodosulfuron-mesosulfuron would have provoked a selection on P450 genes that conduced a convergence of P450-metabolism based resistant Lolium spp. populations, which was detected by markers in a contribution to elucidate the molecular basis of this type of resistance. ; Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-462X |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2020.600301/fullTest; http://hdl.handle.net/11336/172256Test; Yanniccari, Marcos Ezequiel; Gigón, Ramón; Larsen, Adelina Olga; Cytochrome P450 Herbicide Metabolism as the Main Mechanism of Cross-Resistance to ACCase- and ALS-Inhibitors in Lolium spp. Populations From Argentina: A Molecular Approach in Characterization and Detection; Frontiers Media; Frontiers in Plant Science; 11; 11-2020; 1-9; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.3389/fpls.2020.600301Test http://hdl.handle.net/11336/172256Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/2.5/arTest/ |
| رقم الانضمام: | edsbas.822D1D10 |
| قاعدة البيانات: | BASE |
| تدمد: | 1664462X |
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