التفاصيل البيبلوغرافية
| العنوان: |
Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels |
| المؤلفون: |
P Secchiero, D Gibellini, L Flamand, I Robuffo, M Marchisio, S Capitani, R C Gallo, G Zauli |
| المساهمون: |
Secchiero, P, Gibellini, D, Flamand, L, Robuffo, I, Marchisio, M, Capitani, S, C Gallo, R, Zauli, G |
| سنة النشر: |
1997 |
| المجموعة: |
Università degli Studi di Verona: Catalogo dei Prodotti della Ricerca (IRIS) |
| مصطلحات موضوعية: |
HHV-7, CD4, p65lck |
| الوصف: |
In this study, we investigated the fate of the CD4 Ag during infection of CD4+ T cells with the T lymphotrophic human herpesvirus 7 (HHV-7), using the SupT1 lymphoblastoid T cell line as a model system. The following points were established: 1) productive infection with HHV-7 was required to obtain persistent down-modulation of surface CD4 (CD4SURF); 2) at 6 to 9 days postinfection, when approximately 50 to 60% of SupT1 cells still showed a CD4SURF dim positivity, a drastic loss of total CD4 protein was found by either Western blot or immunoprecipitation experiments; 3) a block in CD4 protein production was demonstrated by a radioimmunoprecipitation assay; 4) analysis of the mRNA steady-state levels and transfection studies performed with a plasmid containing the CD4 promoter/enhancer regions in front of the luciferase gene indicated that HHV-7 infection has a suppressive effect on CD4 transcription; 5) both CD4SURF and intracellular CD4 (CD4INTRA) were reduced in HHV-7-infected cells with respect to uninfected controls, but the loss of CD4SURF was more dramatic than that of CD4INTRA; 6) immunogold labeling and electron microscopy demonstrated that CD4INTRA co-localized with HHV-7 Ags within the same subcellular compartments of infected cells; and 7) the total amount of the tyrosine kinase p56lck and tyrosine phosphorylated p56lck levels were unchanged in HHV-7-infected versus uninfected cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
volume:159; issue:7; firstpage:3412; lastpage:3423; numberofpages:12; journal:JOURNAL OF IMMUNOLOGY; https://hdl.handle.net/11562/1078884Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0031253959 |
| الإتاحة: |
https://hdl.handle.net/11562/1078884Test |
| رقم الانضمام: |
edsbas.8204B9D6 |
| قاعدة البيانات: |
BASE |
