دورية أكاديمية
Single cell and spatial sequencing define processes by which keratinocytes and fibroblasts amplify inflammatory responses in psoriasis.
| العنوان: | Single cell and spatial sequencing define processes by which keratinocytes and fibroblasts amplify inflammatory responses in psoriasis. |
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| المؤلفون: | Ma, Feiyang, Plazyo, Olesya, Billi, Allison C, Tsoi, Lam C, Xing, Xianying, Wasikowski, Rachael, Gharaee-Kermani, Mehrnaz, Hile, Grace, Jiang, Yanyun, Harms, Paul W, Xing, Enze, Kirma, Joseph, Xi, Jingyue, Hsu, Jer-En, Sarkar, Mrinal K, Chung, Yutein, Di Domizio, Jeremy, Gilliet, Michel, Ward, Nicole L, Maverakis, Emanual, Klechevsky, Eynav, Voorhees, John J, Elder, James T, Lee, Jun Hee, Kahlenberg, J Michelle, Pellegrini, Matteo, Modlin, Robert L, Gudjonsson, Johann E |
| المصدر: | Nature communications, vol 14, iss 1 |
| بيانات النشر: | eScholarship, University of California |
| سنة النشر: | 2023 |
| المجموعة: | University of California: eScholarship |
| مصطلحات موضوعية: | Fibroblasts, Keratinocytes, Skin, Humans, Psoriasis, Epidermal Cells, Clinical Research, Rare Diseases, Autoimmune Disease, 2.1 Biological and endogenous factors, Aetiology, Inflammatory and immune system |
| الوقت: | 3455 |
| الوصف: | The immunopathogenesis of psoriasis, a common chronic inflammatory disease of the skin, is incompletely understood. Here we demonstrate, using a combination of single cell and spatial RNA sequencing, IL-36 dependent amplification of IL-17A and TNF inflammatory responses in the absence of neutrophil proteases, which primarily occur within the supraspinous layer of the psoriatic epidermis. We further show that a subset of SFRP2+ fibroblasts in psoriasis contribute to amplification of the immune network through transition to a pro-inflammatory state. The SFRP2+ fibroblast communication network involves production of CCL13, CCL19 and CXCL12, connected by ligand-receptor interactions to other spatially proximate cell types: CCR2+ myeloid cells, CCR7+ LAMP3+ dendritic cells, and CXCR4 expressed on both CD8+ Tc17 cells and keratinocytes, respectively. The SFRP2+ fibroblasts also express cathepsin S, further amplifying inflammatory responses by activating IL-36G in keratinocytes. These data provide an in-depth view of psoriasis pathogenesis, which expands our understanding of the critical cellular participants to include inflammatory fibroblasts and their cellular interactions. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | qt8rs9x2v9; https://escholarship.org/uc/item/8rs9x2v9Test; https://escholarship.org/content/qt8rs9x2v9/qt8rs9x2v9.pdfTest |
| DOI: | 10.1038/s41467-023-39020-4 |
| الإتاحة: | https://doi.org/10.1038/s41467-023-39020-4Test https://escholarship.org/uc/item/8rs9x2v9Test https://escholarship.org/content/qt8rs9x2v9/qt8rs9x2v9.pdfTest |
| حقوق: | public |
| رقم الانضمام: | edsbas.81AAA544 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-39020-4 |
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