كتاب
Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities
| العنوان: | Viral Metagenomics Approaches for High-Resolution Screening of Multiplexed Arthropod and Plant Viral Communities |
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| المؤلفون: | François, Sarah, Filloux, Denis, Fernandez, Emmanuel, Ogliastro, Marie Helene, Roumagnac, Philippe |
| المساهمون: | Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
| المصدر: | Viral Metagenomics: Methods and Protocols ; https://hal.science/hal-02154687Test ; Viral Metagenomics: Methods and Protocols, 1746, Humana Press Inc., 217 p., 2018, Methods in Molecular Biology, 978-1-4939-7683-6; 978-1-4939-7682-9. ⟨10.1007/978-1-4939-7683-6_7⟩ |
| بيانات النشر: | HAL CCSD Humana Press Inc. |
| سنة النشر: | 2018 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | Metagenomics, Virus discovery, Diagnostic, Arthropod, Plant, Random amplification, High-throughput sequencing, arthropods, pathologie végétale, maladie virale, arthropode, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this approach, up to 96 arthropod or plant samples can be multiplexed in a single HTS library. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-1-4939-7682-9 1-4939-7682-6 978-1-4939-7683-6 978-0-00-456436-4 1-4939-7683-4 0-00-456436-7 |
| العلاقة: | hal-02154687; https://hal.science/hal-02154687Test; PRODINRA: 459145; WOS: 000456436700008 |
| DOI: | 10.1007/978-1-4939-7683-6_7 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-7683-6_7Test https://hal.science/hal-02154687Test |
| رقم الانضمام: | edsbas.8164D2D4 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781493976829 1493976826 9781493976836 9780004564364 1493976834 0004564367 |
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| DOI: | 10.1007/978-1-4939-7683-6_7 |