تقرير
The tropical coral Pocillopora acuta has a mosaic DNA methylome, an unusual chromatin structure and shows histone H3 clipping
| العنوان: | The tropical coral Pocillopora acuta has a mosaic DNA methylome, an unusual chromatin structure and shows histone H3 clipping |
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| المؤلفون: | Roquis, David, Picart Picolo, Ariadna, Raffalli, Kelly, Romans, Pascal, Masanet, Patrick, Cosseau, Céline, Mitta, Guillaume, Grunau, Christoph, Vidal-Dupiol, Jeremie |
| المساهمون: | Interactions Hôtes-Pathogènes-Environnements (IHPE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), Université de Perpignan Via Domitia (UPVD), Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Aquarium de Canet-en-Roussillon |
| المصدر: | https://hal.archives-ouvertes.fr/hal-03239798Test ; 2021. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2021 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | Pocillopora acuta, Pocillopora damicornis, coral epigenetic, chromatin structure, mosaic DNA methylation, Histone H3 clipping, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] |
| الوصف: | Pocillopora acuta is a hermatypic coral with a worldwide distribution and a strong ecological importance. Anthropogenic disturbances and global warming threaten it. Thermal stress can induce coral bleaching, a phenomenon in which the mutualistic symbiosis between the coral polyps host and its endosymbiotic unicellular algae is disrupted, and can lead to the death of entire colonies. Previous works have shown that soma clonal colonies display different levels of survival depending on the environmental conditions they previously faced. Epigenetic mechanisms are good candidates to explain this phenomenon. The clonal nature of a colony and the possibility of generating genetically identical colonies through propagation make corals an attractive model to study the impact of the environment on the epigenome. However, until now, no work had been published on the P. acuta epigenome. One of the main problems is caused by the intracellular location of Symbiodinium, which makes it complicated to isolate coral chromatin free of contamination by endiosymbiotic biological material. Here, (i) we describe a simple method to purify P. acuta chromatin, (ii) we provide the first description of a coral methylome, with a mosaic pattern of cytosine methylation principally in a CpG context (4% of all CpG), and (iii) we show that P. acuta , but not all corals, has an unusual chromatin structure, and displays histone H3 clipping. |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | hal-03239798; https://hal.archives-ouvertes.fr/hal-03239798Test; https://hal.archives-ouvertes.fr/hal-03239798/documentTest; https://hal.archives-ouvertes.fr/hal-03239798/file/Roquis-2019-BioRxiv-Tropical-OA.pdfTest; BIORXIV: 722322 |
| DOI: | 10.1101/722322 |
| الإتاحة: | https://doi.org/10.1101/722322Test https://hal.archives-ouvertes.fr/hal-03239798Test https://hal.archives-ouvertes.fr/hal-03239798/documentTest https://hal.archives-ouvertes.fr/hal-03239798/file/Roquis-2019-BioRxiv-Tropical-OA.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.8158B11E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/722322 |
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