دورية أكاديمية
Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments
| العنوان: | Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments |
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| المؤلفون: | Grigorian, Eugénie, Groisillier, Agnès, Thomas, François, Leblanc, Catherine, Delage, Ludovic |
| المساهمون: | Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1664-302X. |
| بيانات النشر: | HAL CCSD Frontiers Media |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | L-2-haloacid dehalogenase, Bacteroidetes, haloacid catabolism, evolution, knockout mutant, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; L-2-halocid dehalogenases (L-2-HADs) have been mainly characterized from terrestrial polluted environments. By contrast, knowledge is still scarce about their role in detoxification of predominant halocarbons in marine environments. Here, phylogenetic analyses showed a wide diversity of homologous L-2-HADs, especially among those belonging to marine bacteria. Previously characterized terrestrial L-2-HADs were part of a monophyletic group (named group A) including proteins of terrestrial and marine origin. Another branch (named group B) contained mostly marine L-2-HADs, with two distinct clades of Bacteroidetes homologs, closely linked to Proteobacteria ones. This study further focused on the characterization of the only L-2-HAD from the flavobacterium Zobellia galactanivorans Dsij T (ZgHAD), belonging to one of these Group B clades. The recombinant ZgHAD was shown to dehalogenate bromo- and iodoacetic acids, and gene knockout in Z. galactanivorans revealed a direct role of ZgHAD in tolerance against both haloacetic acids. Analyses of metagenomic and metatranscriptomic datasets confirmed that L-2-HADs from group A were well-represented in terrestrial and marine bacteria, whereas ZgHAD homologs (group B L-2-HADs) were mainly present in marine bacteria, and particularly in host-associated species. Our results suggest that ZgHAD homologs could be key enzymes for marine Bacteroidetes , by conferring selective advantage for the recycling of toxic halogen compounds produced in particular marine habitats, and especially during interactions with macroalgae. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03357342; https://hal.science/hal-03357342Test; https://hal.science/hal-03357342/documentTest; https://hal.science/hal-03357342/file/2021_FiM_Grigorian_ZgalHAD.pdfTest |
| DOI: | 10.3389/fmicb.2021.725997 |
| الإتاحة: | https://doi.org/10.3389/fmicb.2021.725997Test https://hal.science/hal-03357342Test https://hal.science/hal-03357342/documentTest https://hal.science/hal-03357342/file/2021_FiM_Grigorian_ZgalHAD.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.80EA4B7F |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2021.725997 |
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