دورية أكاديمية
Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy
| العنوان: | Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy |
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| المؤلفون: | Bosch, Carles, Ackels, Tobias, Pacureanu, Alexandra, Zhang, Yuxin, Peddie, Christopher J, Berning, Manuel, Rzepka, Norman, Zdora, Marie-Christine, Whiteley, Isabell, Storm, Malte, Bonnin, Anne, Rau, Christoph, Margrie, Troy, Collinson, Lucy, Schaefer, Andreas T |
| المصدر: | Nature Communications , 13 (1) , Article 2923. (2022) |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Animals, Brain, Diffusion Magnetic Resonance Imaging, Imaging, Three-Dimensional, Mice, Microscopy, Electron, Scanning, Synchrotrons, X-Ray Microtomography |
| الوصف: | Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://discovery.ucl.ac.uk/id/eprint/10149852/1/Schaefer_s41467-022-30199-6.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10149852Test/ |
| الإتاحة: | https://discovery.ucl.ac.uk/id/eprint/10149852/1/Schaefer_s41467-022-30199-6.pdfTest https://discovery.ucl.ac.uk/id/eprint/10149852Test/ |
| حقوق: | open |
| رقم الانضمام: | edsbas.80634995 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |