دورية أكاديمية
Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes
| العنوان: | Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes |
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| المؤلفون: | BUSATO, ALICE, Bonafede, Roberta, Bontempi, Pietro, SCAMBI, Ilaria, Schiaffino, Lorenzo, BENATI, Donatella, MALATESTA, Manuela, SBARBATI, Andrea, MARZOLA, Pasquina, MARIOTTI, Raffaella |
| المساهمون: | Busato, Alice, Bonafede, Roberta, Bontempi, Pietro, Scambi, Ilaria, Schiaffino, Lorenzo, Benati, Donatella, Malatesta, Manuela, Sbarbati, Andrea, Marzola, Pasquina, Mariotti, Raffaella |
| سنة النشر: | 2016 |
| المجموعة: | Università degli Studi di Verona: Catalogo dei Prodotti della Ricerca (IRIS) |
| مصطلحات موضوعية: | MRI, superparamagnetic iron oxide nanoparticles, cellular imaging, stem cells labeling, exosome labeling |
| الوصف: | Purpose: Recent findings indicate that the beneficial effects of adipose stem cells (ASCs), reported in several neurodegenerative experimental models, could be due to their paracrine activity mediated by the release of exosomes. The aim of this study was the development and validation of an innovative exosome-labeling protocol that allows to visualize them with magnetic resonance imaging (MRI).Materials and methods: At first, ASCs were labeled using ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, 4–6 nm), and optimal parameters to label ASCs in terms of cell viability, labeling efficiency, iron content, and magnetic resonance (MR) image contrast were investigated. Exosomes were then isolated from labeled ASCs using a standard isolation protocol. The efficiency of exosome labeling was assessed by acquiring MR images in vitro and in vivo as well as by determining their iron content. Transmission electron microscopy images and histological analysis were performed to validate the results obtained.Results: By using optimized experimental parameters for ASC labeling (200 μg Fe/mL of USPIO and 72 hours of incubation), it was possible to label 100% of the cells, while their viability remained comparable to unlabeled cells; the detection limit of MR images was of 102 and 2.5×103 ASCs in vitro and in vivo, respectively. Exosomes isolated from previously labeled ASCs retain nanoparticles, as demonstrated by transmission electron microscopy images. The detection limit by MRI was 3 μg and 5 μg of exosomes in vitro and in vivo, respectively.Conclusion: We report a new approach for labeling of exosomes by USPIO that allows detection by MRI while preserving their morphology and physiological characteristics. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/27330291; info:eu-repo/semantics/altIdentifier/wos/WOS:000377086000001; volume:11; firstpage:2481; lastpage:2490; numberofpages:10; journal:INTERNATIONAL JOURNAL OF NANOMEDICINE; http://hdl.handle.net/11562/944325Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84971517018; http://dx.doi.org/10.2147/IJN.S104152Test |
| DOI: | 10.2147/IJN.S104152 |
| الإتاحة: | https://doi.org/10.2147/IJN.S104152Test http://hdl.handle.net/11562/944325Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.804A97D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2147/IJN.S104152 |
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