دورية أكاديمية
Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor
| العنوان: | Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor |
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| المؤلفون: | Hannon, C, Cruz-Migoni, A, Platonova, O, Owen, R, Nettleship, J, Miller, A, Carr, S, Harris, G, Rabbitts, T, Phillips, S |
| بيانات النشر: | International Union of Crystallography |
| سنة النشر: | 2018 |
| المجموعة: | Oxford University Research Archive (ORA) |
| الوصف: | Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed in Escherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05 Å. The crystals belonged to space group P21, with eight polypeptide chains in the asymmetric unit arranged as an unusual octamer composed of four domain-swapped IBD dimers. IBD exists as a mixture of monomers and dimers in concentrated solutions, but the dimers are unlikely to be biologically relevant. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://ora.ox.ac.uk/objects/uuid:0ff72385-6801-4db4-90b4-547106196781Test; https://doi.org/10.1107/S2053230X18001553Test |
| DOI: | 10.1107/S2053230X18001553 |
| الإتاحة: | https://doi.org/10.1107/S2053230X18001553Test https://ora.ox.ac.uk/objects/uuid:0ff72385-6801-4db4-90b4-547106196781Test |
| حقوق: | info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| رقم الانضمام: | edsbas.7EDFAADD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1107/S2053230X18001553 |
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