دورية أكاديمية
Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro
| العنوان: | Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro |
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| المؤلفون: | Shizuka Takaku, Masami Tsukamoto, Naoko Niimi, Hideji Yako, Kazunori Sango |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 2971, p 2971 (2021) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | glucagon-like peptide-1 receptor, exendin-4, IFRS1 Schwann cells, survival, migration, dorsal root ganglion neurons, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Besides its insulinotropic actions on pancreatic β cells, neuroprotective activities of glucagon-like peptide-1 (GLP-1) have attracted attention. The efficacy of a GLP-1 receptor (GLP-1R) agonist exendin-4 (Ex-4) for functional repair after sciatic nerve injury and amelioration of diabetic peripheral neuropathy (DPN) has been reported; however, the underlying mechanisms remain unclear. In this study, the bioactivities of Ex-4 on immortalized adult rat Schwann cells IFRS1 and adult rat dorsal root ganglion (DRG) neuron–IFRS1 co-culture system were investigated. Localization of GLP-1R in both DRG neurons and IFRS1 cells were confirmed using knockout-validated monoclonal Mab7F38 antibody. Treatment with 100 nM Ex-4 significantly enhanced survival/proliferation and migration of IFRS1 cells, as well as stimulated the movement of IFRS1 cells toward neurites emerging from DRG neuron cell bodies in the co-culture with the upregulation of myelin protein 22 and myelin protein zero. Because Ex-4 induced phosphorylation of serine/threonine-specific protein kinase AKT in these cells and its effects on DRG neurons and IFRS1 cells were attenuated by phosphatidyl inositol-3′-phosphate-kinase (PI3K) inhibitor LY294002, Ex-4 might act on both cells to activate PI3K/AKT signaling pathway, thereby promoting myelination in the co-culture. These findings imply the potential efficacy of Ex-4 toward DPN and other peripheral nerve lesions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/22/6/2971Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/70f411c16de04480a23f15f2b229c7dbTest |
| DOI: | 10.3390/ijms22062971 |
| الإتاحة: | https://doi.org/10.3390/ijms22062971Test https://doaj.org/article/70f411c16de04480a23f15f2b229c7dbTest |
| رقم الانضمام: | edsbas.7E437CE0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms22062971 |