دورية أكاديمية
Fast and cost-effective protocol to produce Paracoccidioides spp. antigens ; Protocolo rápido y económico para la producción de antígenos de Paracoccidioides spp.
| العنوان: | Fast and cost-effective protocol to produce Paracoccidioides spp. antigens ; Protocolo rápido y económico para la producción de antígenos de Paracoccidioides spp. |
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| المؤلفون: | Fernandes-Beraldo, Karolina Rosa, Santos de Freitas-Xavier, Roseli, Pardini-Vicentini, Adriana |
| المصدر: | Biomedica; Vol. 43 No. Sp. 1 (2023): Agosto, Micología médica; 170-180 ; Biomédica; Vol. 43 Núm. Sp. 1 (2023): Agosto, Micología médica; 170-180 ; 2590-7379 ; 0120-4157 |
| بيانات النشر: | Instituto Nacional de Salud |
| سنة النشر: | 2023 |
| المجموعة: | Biomédica - Revista del Instituto Nacional de Salud, Bogotá |
| مصطلحات موضوعية: | Paracoccidioides, paracoccidioidomycosis, antigens, immunologic tests, paracoccidioidomicosis, antígenos, pruebas inmunológicas |
| الوصف: | Introduction. The existing methods for Paracoccidioides spp. antigen production are problematic in terms of standardization, specificity, stability, repeatability, and reproducibility.Objective. To optimize the methodology for Paracoccidioides spp. antigen production and evaluate its applicability in paracoccidioidomycosis immunodiagnosis.Materials and methods. The antigens were obtained from Paracoccidioides lutzii isolates (01, 66, and 8334), Paracoccidioides brasiliensis sensu stricto (113), and Paracoccidioides restripiensis (B-339). These fungi were grown at 36 °C ± 1 °C, on modified Fava-Netto agar, according to Freitas et al. (2018). Paracoccidioides lutzii antigens were obtained after , 10, and 20 days of culture, whereas P. brasiliensis and P. restripiensis antigens were obtained after 10 days. Antigens were evaluated in natura, 10 and 20 times concentrated. Antigenic capacity was evaluated using a double immunodiffusion assay against serum samples from patients with paracoccidioidomycosis, histoplasmosis, and aspergillosis, and random blood donors.Results. Cross-reactivity between Paracoccidioides spp. antigens was observed when P. brasiliensis, P. restrepiensis antigens, and P. lutzii antigens were evaluated with the polyclonal antibodies against P. lutzii and P. brasiliensis, respectively. No cross-reactivity was obtained for polyclonal antibodies against Histoplasma capsulatum, Aspergillus fumigatus, and random blood donors. The proposed protocol allowed stable, repeatable, and reproducible genus-specific antigen production at a low cost and in a short cultivation time.Conclusion. The proposed protocol allowed us to obtain genus-specific antigens that can be developed and reproduced in all laboratories in Brazil and South America, where paracoccidioidomycosis is a neglected disease, contributing to an early diagnosis, especially in endemic regions, regardless of the species. ; Introducción. Los métodos existentes para la producción de los antígenos de Paracoccidioides spp. son problemáticos en su ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; text/xml |
| اللغة: | English |
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| الإتاحة: | https://doi.org/10.1002/yea.3147Test https://doi.org/10.1016/S1473-3099Test(17)30306-7 https://doi.org/10.1590/0037-8682-0230-2017Test https://doi.org/10.3390/jof3010001Test https://doi.org/10.2174/1874285801711010224Test https://doi.org/10.1016/j.diagmicrobio.2015.06.004Test https://doi.org/10.2217/fmb-2016-0062Test https://doi.org/10.3390/jof8100993Test https://doi.org/10.3390/jof8101098Test https://doi.org/10.1186/s40409-017-0101-3Test |
| حقوق: | Derechos de autor 2023 Biomédica ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.7E368D39 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |