دورية أكاديمية
Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3‑Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates
| العنوان: | Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3‑Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates |
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| المؤلفون: | Lei Zhou, Yufei Zhang, Tingting Chen, Junhua Yun, Mei Zhao, Hossain M. Zabed, Cunsheng Zhang, Xianghui Qi |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Molecular Biology, Physiology, Pharmacology, Biotechnology, Ecology, Developmental Biology, Inorganic Chemistry, Infectious Diseases, Computational Biology, Space Science, Chemical Sciences not elsewhere classified, key platform chemical, derived substrates embracing, corn stover bioprocessing, convert cellulosic hydrolysate, cellulosic hydrolysate, corn stover, convert glycerol, sustainable development, successfully established, p phosphatase, p dehydrogenase, metabolically rewired, metabolically modulated, metabolic remodulation, hydroxypropionic acid, heterologous pathways, gpdp < |
| الوصف: | Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, namely, 3-hydroxypropionic acid (3-HP). First, the heterologous pathways were introduced into E. coli by coexpressing glycerol-3-P dehydrogenase and glycerol-3-P phosphatase in both single and fusion ( gpdp 12) forms, making the strain capable of synthesizing glycerol from glucose. Subsequently, a glycerol dehydratase (DhaB123-gdrAB) and an aldehyde dehydrogenase (GabD4) were overexpressed to convert glycerol into 3-HP. A fine-tuning between glycerol synthesis and its conversion into 3-HP was successfully established by 5′-untranslated region engineering of gpdp 12 and dhaB 123- gdrAB . The strain was further metabolically modulated to successfully prevent glycerol flux outside the cell and into the central metabolism. The finally remodulated chassis produced 32.91 g/L 3-HP from the cellulosic hydrolysate of stover during fed-batch fermentation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Metabolic_Remodulation_of_Chassis_and_Corn_Stover_Bioprocessing_to_Unlock_3_Hydroxypropionic_Acid_Biosynthesis_from_Agrowaste-Derived_Substrates/25049861Test |
| DOI: | 10.1021/acs.jafc.3c08419.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jafc.3c08419.s001Test https://figshare.com/articles/journal_contribution/Metabolic_Remodulation_of_Chassis_and_Corn_Stover_Bioprocessing_to_Unlock_3_Hydroxypropionic_Acid_Biosynthesis_from_Agrowaste-Derived_Substrates/25049861Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.7D3BA1A2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jafc.3c08419.s001 |
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