دورية أكاديمية
Insulin-like growth factor-I is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2
| العنوان: | Insulin-like growth factor-I is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2 |
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| المؤلفون: | Arsenijevic, Y., Weiss, S., Schneider, B., Aebischer, P. |
| المصدر: | Journal of Neuroscience, vol. 21, no. 18, pp. 7194-202 |
| سنة النشر: | 2001 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Animals Autocrine Communication/drug effects/physiology Cell Count Cell Division/drug effects Cell Survival/drug effects Cells, Cultured Corpus Striatum Dose-Response Relationship, Drug Epidermal Growth Factor/*pharmacology Fibroblast Growth Factor 2/*pharmacology Insulin-Like Growth Factor I/*pharmacology Mice Neurons/cytology/*drug effects/metabolism Spheroids, Cellular/cytology/drug effects Stem Cells/cytology/*drug effects/metabolism |
| الوصف: | Neural stem cells (NSCs), when stimulated with epidermal growth factor (EGF) or fibroblast growth factor-2 (FGF-2), have the capacity to renew, expand, and produce precursors for neurons, astrocytes, and oligodendrocytes. We postulated that the early appearance of insulin-like growth factor (IGF-I) receptors during mouse striatum development implies a role in NSC regulation. Thus, we tested in vitro the action of IGF-I on the proliferation of striatal NSCs. In the absence of IGF-I, neither EGF nor FGF-2 was able to induce the proliferation of E14 mouse striatal cells. However, addition of IGF-I generated large proliferative clusters, termed spheres, in a dose-dependent manner. The newly generated spheres were multipotent, and clonal analysis revealed that EGF or FGF-2, in the presence of IGF-I, acted directly on NSCs. The actions of IGF-I suggest distinct modes of action of EGF or FGF-2 on NSCs. First, continuous versus delayed administration of these neurotrophic factors showed that neither IGF-I nor EGF had an effect on NSC survival, whereas FGF-2 promoted the survival or maintenance of the stem cell state of 50% of NSCs for 6 d. Second, short-term exposure to IGF-I induced the proliferation of NSCs in the presence of EGF, but not of FGF-2, through an autocrine secretion of IGF-I. These findings suggest that IGF-I is a key factor in the regulation of NSC activation and that EGF and FGF-2 control striatal NSC proliferation, in part, through distinct intracellular mechanisms. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/11549730; info:eu-repo/semantics/altIdentifier/eissn/1529-2401; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_755860E86E1F7; https://serval.unil.ch/notice/serval:BIB_755860E86E1FTest; https://serval.unil.ch/resource/serval:BIB_755860E86E1F.P001/REF.pdfTest; http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_755860E86E1F7Test |
| الإتاحة: | https://serval.unil.ch/notice/serval:BIB_755860E86E1FTest https://serval.unil.ch/resource/serval:BIB_755860E86E1F.P001/REF.pdfTest http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_755860E86E1F7Test |
| حقوق: | info:eu-repo/semantics/openAccess ; Copying allowed only for non-profit organizations ; https://serval.unil.ch/disclaimerTest |
| رقم الانضمام: | edsbas.7CC85F20 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |