دورية أكاديمية
Preparation of Well-Defined Fluorescent Nanoplastic Particles by Confined Impinging Jet Mixing
| العنوان: | Preparation of Well-Defined Fluorescent Nanoplastic Particles by Confined Impinging Jet Mixing |
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| المؤلفون: | Livius F. Muff, Sandor Balog, Jozef Adamcik, Christoph Weder, Roman Lehner |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Space Science, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, biological end points, broadly applicable method, different np types, defined reference materials, different types, readily applicable, different systems, different fluorophores, tailorable characteristics, still missing, postfabrication process, possible impact, polymer nanoparticles, isolated reports, human health, heterogeneous geometries, complex environments, broad range, 100 nm, 1 macrophages |
| الوصف: | Research on the origin, distribution, detection, identification, and quantification of polymer nanoparticles (NPs) in the environment and their possible impact on animal and human health is surging. For different types of studies in this field, well-defined reference materials or mimics are needed. While isolated reports on the preparation of such materials are available, a simple and broadly applicable method that allows for the production of different NP types with well-defined, tailorable characteristics is still missing. Here, we demonstrate that a confined impinging jet mixing process can be used to prepare colloidally stable NPs based on polystyrene, polyethylene, polypropylene, and poly(ethylene terephthalate) with diameters below < 100 nm. Different fluorophores were incorporated into the NPs, to allow their detection in complex environments. To demonstrate their utility and detectability, fluorescent NPs were exposed to J774A.1 macrophages and visualized using laser scanning microscopy. Furthermore, we modified the NPs in a postfabrication process and changed their shape from spherical to heterogeneous geometries, in order to mimic environmentally relevant morphologies. The methodology used here should be readily applicable to other polymers and payloads and thus a broad range of NPs that enable studies of their behavior, uptake, translocation, and biological end points in different systems. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Preparation_of_Well-Defined_Fluorescent_Nanoplastic_Particles_by_Confined_Impinging_Jet_Mixing/24477137Test |
| DOI: | 10.1021/acs.est.3c03697.s002 |
| الإتاحة: | https://doi.org/10.1021/acs.est.3c03697.s002Test https://figshare.com/articles/journal_contribution/Preparation_of_Well-Defined_Fluorescent_Nanoplastic_Particles_by_Confined_Impinging_Jet_Mixing/24477137Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.7C678F1C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.est.3c03697.s002 |
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