التفاصيل البيبلوغرافية
| العنوان: |
Evaluating the reproducibility of SBCapSeq results from bulk cuSCC and normal skin specimens. |
| المؤلفون: |
Aziz Aiderus (5618408), Justin Y. Newberg (11278931), Liliana Guzman-Rojas (47811), Ana M. Contreras-Sandoval (11278934), Amanda L. Meshey (11278937), Devin J. Jones (11278940), Felipe Amaya-Manzanares (374499), Roberto Rangel (47810), Jerrold M. Ward (11278943), Song-Choon Lee (11278946), Kenneth Hon-Kim Ban (11278949), Keith Rogers (257548), Susan M. Rogers (11278952), Luxmanan Selvanesan (11278955), Leslie A. McNoe (11278958), Neal G. Copeland (11278961), Nancy A. Jenkins (11278964), Kenneth Y. Tsai (1349253), Michael A. Black (11278967), Karen M. Mann (11278970), Michael B. Mann (11278973) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Molecular Biology, Cancer, Hematology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, cuSCC, novel oncogenic drivers, NCOA, 2C, Sleeping Beauty transposon mutagene., cell carcinoma progression, UV-IR, landscape, skin oncogenic etiology, ZMIZ, cutaneou, absence, tumor suppressor genes, initiation, tumor suppressive roles, KMT, vivo, oncogenic driver mutation, keratinocyte transformation, CREBBP |
| الوصف: |
Representative plots of individual SB insertion sites based on read depth from two individual cuSCC genomes ( A-B ) and two unselected skin cell genomes from histologically normal skin ( C-D ) using genomic DNAs isolated from bulk tumor specimens from biological replicate libraries (independent library workflows applied to the same biological specimen isolate) comparing library 1 ( x -axis) to library 2 ( y -axis). Biological reproducibility of cuSCC specimen libraries is indicated by both Pearson’s and Spearman’s correlation metrics ( A-B ). No biological reproducibility of normal skin specimen libraries is observed ( C-D ) indicated by negative values for Pearson’s and Spearman’s correlation metrics and a failure to identify the same SB insertion sites. Individual SB insertion sites plotted by read depth from two technical replicates (the same library preparation) from two separate sequencing runs ( E-F ) confirms high reproducibility between individual libraries at high-to-moderate read depths, supported by both Pearson’s (r = 0.48) and Spearman’s (ρ = 0.49 or undetermined) correlation metrics. (TIFF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Evaluating_the_reproducibility_of_SBCapSeq_results_from_bulk_cuSCC_and_normal_skin_specimens_/15175280Test |
| DOI: |
10.1371/journal.pgen.1009094.s003 |
| الإتاحة: |
https://doi.org/10.1371/journal.pgen.1009094.s003Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.7BD70C00 |
| قاعدة البيانات: |
BASE |
