دورية أكاديمية
Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes
| العنوان: | Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes |
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| المؤلفون: | Callaghan, Neal I., Lee, Shin-Haw, Hadipour-Lakmehsari, Sina, Lee, Xavier A., Ahsan Siraj, M., Driouchi, Amine, Yip, Christopher M., Husain, Mansoor, Simmons, Craig A., Gramolini, Anthony O. |
| المصدر: | Communications Biology ; volume 3, issue 1 ; ISSN 2399-3642 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| الوصف: | Primary adult cardiomyocyte (aCM) represent the mature form of myocytes found in the adult heart. However, culture of aCMs in particular is challenged by poor survival and loss of phenotype, rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that enhance survival and maintain sarcomeric structure and Ca 2+ cycling to enable physiologically relevant contractile force measurements. We also demonstrate genetic and small-molecule manipulations that probe mechanisms underlying myocyte functional performance. Together, these refinements to aCM culture present a toolbox with which to advance our understanding of myocardial physiology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s42003-020-0946-9 |
| الإتاحة: | https://doi.org/10.1038/s42003-020-0946-9Test https://www.nature.com/articles/s42003-020-0946-9.pdfTest https://www.nature.com/articles/s42003-020-0946-9Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.7B136F5D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s42003-020-0946-9 |
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