دورية أكاديمية
Ki20227 aggravates apoptosis, inflammatory response, and oxidative stress after focal cerebral ischemia injury
| العنوان: | Ki20227 aggravates apoptosis, inflammatory response, and oxidative stress after focal cerebral ischemia injury |
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| المؤلفون: | Cheng Jiang, Ze-Ning Wang, Yu-Chen Kang, Yi Chen, Wei-Xin Lu, Hai-Jun Ren, Bo-Ru Hou |
| المصدر: | Neural Regeneration Research, Vol 17, Iss 1, Pp 137-143 (2022) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | apoptosis, colony-stimulating factor-1 receptor, inflammatory response, ischemia/reperfusion, ki20227, microglia, oxidative stress, transient middle cerebral artery occlusion, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | The survival of microglia depends on the colony-stimulating factor-1 receptor (CSF1R) signaling pathway under physiological conditions. Ki20227 is a highly selective CSF1R inhibitor that has been shown to change the morphology of microglia. However, the effects of Ki20227 on the progression of ischemic stroke are unclear. In this study, male C57BL/6 mouse models of focal cerebral ischemic injury were established through the occlusion of the middle cerebral artery and then administered 3 mg/g Ki20227 for 3 successive days. The results revealed that the number of ionized calcium-binding adaptor molecule 1/bromodeoxyuridine double positive cells in the infarct tissue was reduced, the degree of edema was increased, neurological deficits were aggravated, infarct volume was increased, and the number of peri-infarct Nissl bodies was reduced. The number of terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells in the peri-infarct tissue was increased. The expression levels of Bax and Cleaved caspase-3 were up-regulated. Bcl-2 expression was downregulated. The expression levels of inflammatory factors and oxidative stress-associated factors were increased. These findings suggested that Ki20227 blocked microglial proliferation and aggravated the pathological progression of ischemia/reperfusion injury in a transient middle cerebral artery occlusion model. This study was approved by the Animal Ethics Committee of Lanzhou University Second Hospital (approval No. D2020-68) on March 6, 2020. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1673-5374 |
| العلاقة: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=137;epage=143;aulast=JiangTest; https://doaj.org/toc/1673-5374Test; https://doaj.org/article/6b6b1c84253b48d6a42b6e7296ca9c3dTest |
| DOI: | 10.4103/1673-5374.314318 |
| الإتاحة: | https://doi.org/10.4103/1673-5374.314318Test https://doaj.org/article/6b6b1c84253b48d6a42b6e7296ca9c3dTest |
| رقم الانضمام: | edsbas.7AF6C586 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16735374 |
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| DOI: | 10.4103/1673-5374.314318 |