التفاصيل البيبلوغرافية
| العنوان: |
Mucosal-Associated Invariant T Cells are not susceptible in vitro to SARS-CoV-2 infection but accumulate into the lungs of COVID-19 patients |
| المؤلفون: |
Huang, Xiaobo, Kantonen, Jonas, Nowlan, Kirsten, Nguyen, Ngoc Anh, Jokiranta, Suvi T., Kuivanen, Suvi, Heikkilä, Nelli, Mahzabin, Shamita, Kantele, Anu, Vapalahti, Olli, Myllykangas, Liisa, Heinonen, Santtu, Mäyränpää, Mikko I., Strandin, Tomas, Kekäläinen, Eliisa |
| المساهمون: |
TRIMM - Translational Immunology Research Program, Department of Bacteriology and Immunology, HUS Helsinki and Uusimaa Hospital District, HUSLAB, Department of Pathology, HUS Diagnostic Center, Medicum, Viral Zoonosis Research Unit, Department of Virology, Molecular and Integrative Biosciences Research Programme, Department of Medicine, HUS Inflammation Center, Clinicum, HUMI - Human Microbiome Research, Infektiosairauksien yksikkö, Helsinki One Health (HOH), Veterinary Microbiology and Epidemiology, Veterinary Biosciences, Olli Pekka Vapalahti / Principal Investigator, Children's Hospital, HUS Children and Adolescents |
| بيانات النشر: |
Elsevier Scientific Publ. Co |
| سنة النشر: |
2024 |
| المجموعة: |
Helsingfors Universitet: HELDA – Helsingin yliopiston digitaalinen arkisto |
| مصطلحات موضوعية: |
COVID-19, Lymphopenia, Mucosal Associated Invariant T cells, SARS-CoV-2, Viral challenge, 3111 Biomedicine, 11832 Microbiology and virology |
| الوصف: |
Prolonged T cell lymphopenia is common in COVID-19, caused by SARS-CoV-2. While the mechanisms of lymphopenia during COVID-19 remain elusive, it is especially pronounced in a specialized innate-like T cell population called Mucosal Associated Invariant T cells (MAITs). MAITs has been suggested to express Angiotensin-Converting Enzyme 2 (ACE2), which is the well-known cellular receptor for SARS-CoV-2. However, it is still unclear if SARS-CoV-2 can infect or affect MAIT cells directly. In this study, we performed multicolor flow cytometry on peripheral blood mononuclear cells obtained from COVID-19 patients to assess the frequencies of CD8+Vα7.2+CD161+ MAIT subsets at acute and convalescent disease phases. The susceptibility of MAITs and T cells to direct exposure by SARS-CoV-2 was analysed using cells isolated from healthy donor buffy coats by viability assays, virus-specific RT-PCR, and flow cytometry. In situ lung immunofluorescence was used to evaluate retention of T cells, especially MAIT cells, in lung tissues during acute COVID-19. Our study confirms previous reports indicating that circulating MAITs are activated, and their frequency is declined in patients with acute SARS-CoV-2 infection, whereas an accumulation of MAITs and T cells was seen in the lung tissue of individuals with fatal COVID-19. However, despite a fraction of MAITs found to express ACE2, no evidence for the susceptibility of MAITs for direct infection or activation by SARS-CoV-2 particles was observed. Thus, their activation and decline in the circulation is most likely explained by indirect mechanisms involving other immune cells and cytokine-induced pro-inflammatory environment but not by direct exposure to viral particles at the infection site. ; Peer reviewed |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
The samples used for the study were obtained from Helsinki University Hospital, Helsinki, Finland. We thank all the participants in this study. We also thank the excellent technical assistance of laboratory technicians Marjo K. Rissanen, Tamas S. Bazsinka, Sanna Mäki and Kristiina Nokelainen. We acknowledge Simo Miettinen for organising COVID-19 blood sample collection. This study is funded by The Finnish Medical Foundation, Sigrid Juselius Foundation and the Academy of Finland (Grant No's. 1336490 , 336439 and 335527 ). Open access was funded by University of Helsinki Library. The funders of the study had no role in study design, data collection, data analysis, or writing of the report. The samples used for the study were obtained from Helsinki University Hospital, Helsinki, Finland. We thank all the participants in this study. We also thank the excellent technical assistance of laboratory technicians Marjo K. Rissanen, Tamas S. Bazsinka, Sanna Mäki and Kristiina Nokelainen. We acknowledge Simo Miettinen for organising COVID-19 blood sample collection. This study is funded by The Finnish Medical Foundation, Sigrid Juselius Foundation and the Academy of Finland (Grant No's. 1336490, 336439 and 335527). Open access was funded by University of Helsinki Library. The funders of the study had no role in study design, data collection, data analysis, or writing of the report. The MR1 tetramer technology was developed jointly by Dr. James McCluskey, Dr. Jamie Rossjohn, and Dr. David Fairlie, and the material was produced by the NIH Tetramer Core Facility as permitted to be distributed by the University of Melbourne.; Huang , X , Kantonen , J , Nowlan , K , Nguyen , N A , Jokiranta , S T , Kuivanen , S , Heikkilä , N , Mahzabin , S , Kantele , A , Vapalahti , O , Myllykangas , L , Heinonen , S , Mäyränpää , M I , Strandin , T & Kekäläinen , E 2024 , ' Mucosal-Associated Invariant T Cells are not susceptible in vitro to SARS-CoV-2 infection but accumulate into the lungs of COVID-19 patients ' , Virus Research , vol. 341 , 199315 . https://doi.org/10.1016/j.virusres.2024.199315Test; ORCID: /0000-0003-2270-6824/work/153473383; ORCID: /0000-0002-0877-9640/work/153473675; ORCID: /0000-0003-4988-185X/work/153474023; ORCID: /0000-0002-2237-5466/work/153474505; ORCID: /0000-0002-1666-1933/work/153475344; ORCID: /0000-0001-6045-108X/work/153477445; 85182564256; 683e58df-208e-4ee4-83d3-034e6223d7ec; http://hdl.handle.net/10138/571193Test; 001166344500001 |
| الإتاحة: |
http://hdl.handle.net/10138/571193Test |
| حقوق: |
cc_by ; openAccess ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.79B007AF |
| قاعدة البيانات: |
BASE |
