دورية أكاديمية
Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways
| العنوان: | Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways |
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| المؤلفون: | Ding, Wei-Qun, Larsson, Christer, Alling, Christer |
| المصدر: | Journal of Neurochemistry; 70(4), pp 1722-1729 (1998) ; ISSN: 1471-4159 |
| بيانات النشر: | Wiley-Blackwell |
| سنة النشر: | 1998 |
| المجموعة: | Lund University Publications (LUP) |
| مصطلحات موضوعية: | Neurosciences, Muscarinic receptor, Protein kinase C, Immediate-early genes, SH-SY5Y neuroblastoma cells, Ca2+/calmodulin-dependent kinase II |
| الوصف: | The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Comparison of the inhibitory effects of GF109203X and Go 6976 suggests the involvement of classical protein kinase C isozymes in muscarinic receptor-stimulated expression of fos and jun genes. These results demonstrate that the muscarinic receptor-induced expression of individual fos and jun genes is regulated via different pathways, primarily protein kinase C or Ca2+/calmodulin-dependent kinase II. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://lup.lub.lu.se/record/1112630Test; http://dx.doi.org/10.1046/j.1471-4159.1998.70041722.xTest; pmid:9523591; scopus:0031881692 |
| DOI: | 10.1046/j.1471-4159.1998.70041722.x |
| الإتاحة: | https://doi.org/10.1046/j.1471-4159.1998.70041722.xTest https://lup.lub.lu.se/record/1112630Test |
| رقم الانضمام: | edsbas.79727A09 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1471-4159.1998.70041722.x |
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