دورية أكاديمية
Au@Ag Core−Shell Nanoparticles for Colorimetric and Surface- Enhanced Raman-Scattering-Based Multiplex Competitive Lateral Flow Immunoassay for the Simultaneous Detection of Histamine and Parvalbumin in Fish
| العنوان: | Au@Ag Core−Shell Nanoparticles for Colorimetric and Surface- Enhanced Raman-Scattering-Based Multiplex Competitive Lateral Flow Immunoassay for the Simultaneous Detection of Histamine and Parvalbumin in Fish |
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| المؤلفون: | Fernández-Lodeiro, Carlos, González-Cabaleiro, Lara, Vázquez-Iglesias, Lorena, Serrano-Partierra, Esther, Bodelón, Gustavo, Carrera, Mónica, Blanco-López, María Carmen, Pérez-Juste, Jorge, Pastoriza-Santos, Isabel |
| المساهمون: | European Commission, Ministerio de Ciencia e Innovación (España), Xunta de Galicia, Principado de Asturias, Universidad de Vigo, Serrano-Partierra, Esther, Bodelón, Gustavo, Carrera, Mónica, Blanco-López, María Carmen, Pérez-Juste, Jorge, Pastoriza-Santos, Isabel |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2024 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | SERS tags, Multiplex detection, Lateral flow assay, Allergens |
| الوصف: | 11 pages, 2 tables, 5 figures.-- This article is licensed under CC-BY 4.0 ; Foodborne allergies and illnesses represent a major global health concern. In particular, fish can trigger life-threatening food allergic reactions and poisoning effects, mainly caused by the ingestion of parvalbumin toxin. Additionally, preformed histamine in less-than-fresh fish serves as a toxicological alert. Consequently, the analytical assessment of parvalbumin and histamine levels in fish becomes a critical public health safety measure. The multiplex detection of both analytes has emerged as an important issue. The analytical detection of parvalbumin and histamine requires different assays; while the determination of parvalbumin is commonly carried out by enzyme-linked immunosorbent assay, histamine is analyzed by high-performance liquid chromatography. In this study, we present an approach for multiplexing detection and quantification of trace amounts of parvalbumin and histamine in canned fish. This is achieved through a colorimetric and surface-enhanced Raman-scattering-based competitive lateral flow assay (SERS-LFIA) employing plasmonic nanoparticles. Two distinct SERS nanotags tailored for histamine or β-parvalbumin detection were synthesized. Initially, spherical 50 nm Au@Ag core–shell nanoparticles (Au@Ag NPs) were encoded with either rhodamine B isothiocyanate (RBITC) or malachite green isothiocyanate (MGITC). Subsequently, these nanoparticles were bioconjugated with anti-β-parvalbumin and antihistamine, forming the basis for our detection and quantification methodology. Additionally, our approach demonstrates the use of SERS-LFIA for the sensitive and multiplexed detection of parvalbumin and histamine on a single test line, paving the way for on-site detection employing portable Raman instruments ; The authors acknowledge financial support from the European Innovation Council (Horizon 2020 Project: 965018-BIOCELLPHE), the MCIN/AEI/10.13039/501100011033 (grant PID2019-108954RB-I00 and PID2019-103845RB-C21), the FSE (“El ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2574-0970 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108954RB-I00/ES/DISEÑO RACIONAL DE SERS TAGS DE ALTO EFICIENCIA PARA DETECCION MEDIANTE INMUNOENSAYO Y BIOIMAGEN/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103845RB-C21/ES/BIOLOGIA DE SISTEMAS BASADA EN PROTEOMICA Y BIOLOGIA ESTRUCTURAL EN LA ALERGIA AL PESCADO EN PRODUCTOS PESQUEROS FRESCOS Y PROCESADOS/; info:eu-repo/grantAgreement/EC/H2020/965018; Publisher's version; The data that support the findings of this study are available at ZENODO, doi:10.5281/zenodo.10036362; https://doi.org/10.1021/acsanm.3c04696Test; Sí; ACS Applied Nano Materials 7(1): 498–508 (2024); http://hdl.handle.net/10261/343037Test |
| DOI: | 10.1021/acsanm.3c04696 |
| الإتاحة: | https://doi.org/10.1021/acsanm.3c04696Test https://doi.org/10.5281/zenodo.10036362Test http://hdl.handle.net/10261/343037Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.790013E |
| قاعدة البيانات: | BASE |
| تدمد: | 25740970 |
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| DOI: | 10.1021/acsanm.3c04696 |