دورية أكاديمية
Clinical-grade mesenchymal stem cells derived from umbilical cord improve septic shock in pigs
| العنوان: | Clinical-grade mesenchymal stem cells derived from umbilical cord improve septic shock in pigs |
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| المؤلفون: | Laroye, Caroline, Lemarié, Jérémie, Boufenzer, Amir, Labroca, Pierre, Cunat, Lisiane, Alauzet, Corentine, Groubatch, Frederique, Cailac, Clémence, Jolly, Lucie, Bensoussan, Danièle, Reppel, Loïc, Gibot, Sebastien |
| المساهمون: | Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), unité de thérapie cellulaire et Tissus (UTCT), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Service de Réanimation Médicale CHRU Nancy, INOTREM SA, Faculté de Médecine Nancy, Université de Lorraine (UL)-Université de Lorraine (UL), Université de Lorraine (UL), Stress, Immunité, Pathogènes (SIMPA), Ecole de Chirurgie, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Faculté de Médecine, Département d’Anatomie et Cytologie Pathologiques CHRU Nancy |
| المصدر: | ISSN: 2197-425X. |
| بيانات النشر: | HAL CCSD Springer |
| سنة النشر: | 2018 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Background: Septic shock is the leading cause of death in intensive care units. The pathophysiological complexity of this syndrome contributes to an absence of specific treatment. Several preclinical studies in murine models of septic shock have shown improvements to organ injury and survival after administration of mesenchymal stem cells (MSCs). To better mimic a clinical approach in humans, we investigated the impact of randomized controlled double-blind administration of clinical-grade umbilical cord-derived MSCs to a relevant pig model of septic shock.Methods: Septic shock was induced by fecal peritonitis in 12 male domestic pigs. Animals were resuscitated by an experienced intensivist including fluid administration and vasopressors. Four hours after the induction of peritonitis, pigs were randomized to receive intravenous injection of thawed umbilical cord-derived MSCs (UCMSC) (1 × 106 UCMSCs/kg diluted in 75 mL hydroxyethyl starch (HES), (n = 6) or placebo (HES alone, n = 6). Researchers were double-blinded to the treatment administered. Hemodynamic parameters were continuously recorded. Gas exchange, acid-base status, organ function, and plasma cytokine concentrations were assessed at regular intervals until 24 h after the onset of peritonitis when animals were sacrificed under anesthesia.Results: Peritonitis induced profound hypotension, hyperlactatemia, and multiple organ failure. These disorders were significantly attenuated when animals were treated with UCMSCs. In particular, cardiovascular failure was attenuated, as attested by a better mean arterial pressure and reduced lactatemia, despite lower norepinephrine requirements. As such, UCMSCs improved survival in this very severe model (60% survival vs. 0% at 24 h).Conclusion: UCMSCs administration is beneficial in this pig model of polymicrobial septic shock. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30091119; hal-02906219; https://hal.archives-ouvertes.fr/hal-02906219Test; PUBMED: 30091119; PUBMEDCENTRAL: PMC6082751 |
| DOI: | 10.1186/s40635-018-0194-1 |
| الإتاحة: | https://doi.org/10.1186/s40635-018-0194-1Test https://hal.archives-ouvertes.fr/hal-02906219Test |
| رقم الانضمام: | edsbas.761DCEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s40635-018-0194-1 |
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