دورية أكاديمية
Lung epithelial and myeloid innate immunity in influenza-associated or COVID-19-associated pulmonary aspergillosis: an observational study
| العنوان: | Lung epithelial and myeloid innate immunity in influenza-associated or COVID-19-associated pulmonary aspergillosis: an observational study |
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| المؤلفون: | Feys, S., Gonçalves, S.M., Khan, M., Choi, S., Boeckx, B., Chatelain, D., Cunha, C., Debaveye, Y., Hermans, G., Hertoghs, M., Humblet-Baron, S., Jacobs, Cato, Lagrou, K., Marcelis, L., Maizel, J., Meersseman, P., Nyga, R., Seldeslachts, L., Starick, M.R., Thevissen, K., Vandenbriele, C., Vanderbeke, L., Velde, Greetje van de, Regenmortel, N. Van, Vanstapel, A., Vanmassenhove, S., Wilmer, A., Veerdonk, F.L. van de, Hertogh, G. de, Mombaerts, P., Lambrechts, D., Carvalho, A., Weyenbergh, J. Van, Wauters, J. |
| المصدر: | Lancet Respiratory Medicine, 10, 12, pp. 1147-1159 |
| سنة النشر: | 2022 |
| المجموعة: | Radboud University: DSpace |
| مصطلحات موضوعية: | Radboudumc 4: lnfectious Diseases and Global Health RIMLS: Radboud Institute for Molecular Life Sciences |
| الوصف: | Contains fulltext : 286888.pdf (Publisher’s version ) (Closed access) ; BACKGROUND: Influenza-associated pulmonary aspergillosis (IAPA) and COVID-19-associated pulmonary aspergillosis (CAPA) affect about 15% of critically ill patients with influenza or COVID-19, respectively. These viral-fungal coinfections are difficult to diagnose and are associated with increased mortality, but data on their pathophysiology are scarce. We aimed to explore the role of lung epithelial and myeloid innate immunity in patients with IAPA or CAPA. METHODS: In this observational study, we retrospectively recruited patients who had been admitted to the intensive care unit (ICU) of University Hospitals Leuven, Belgium, requiring non-invasive or invasive ventilation because of severe influenza or COVID-19, with or without aspergillosis, between Jan 1, 2011, and March 31, 2021, whose bronchoalveolar lavage samples were available at the hospital biobank. Additionally, biobanked in vivo tracheobronchial biopsy samples from patients with IAPA or CAPA and invasive Aspergillus tracheobronchitis admitted to ICUs requiring invasive ventilation between the same dates were collected from University Hospitals Leuven, Hospital Network Antwerp (Belgium), and Amiens-Picardie University Hospital (France). We did nCounter gene expression analysis of 755 genes linked to myeloid innate immunity and protein analysis of 47 cytokines, chemokines, and growth factors on the bronchoalveolar lavage samples. Gene expression data were used to infer cell fractions by use of CIBERSORTx, to perform hypergeometric enrichment pathway analysis and gene set enrichment analysis, and to calculate pathway module scores for the IL-1β, TNF-α, type I IFN, and type II IFN (IFNγ) pathways. We did RNAScope targeting influenza virus or SARS-CoV-2 RNA and GeoMx spatial transcriptomics on the tracheobronchial biopsy samples. FINDINGS: Biobanked bronchoalveolar lavage samples were retrieved from 166 eligible patients, of whom 40 had IAPA, 52 had influenza without aspergillosis, 33 ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://repository.ubn.ru.nl/handle/2066/286888Test; https://doi.org/10.1016/S2213-2600Test(22)00259-4 |
| DOI: | 10.1016/S2213-2600(22)00259-4 |
| الإتاحة: | https://doi.org/10.1016/S2213-2600Test(22)00259-4 https://repository.ubn.ru.nl/handle/2066/286888Test |
| رقم الانضمام: | edsbas.75C9445D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/S2213-2600(22)00259-4 |
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