دورية أكاديمية
TUNAR lncRNA encodes a microprotein that regulates neural differentiation and neurite formation by modulating calcium dynamics
| العنوان: | TUNAR lncRNA encodes a microprotein that regulates neural differentiation and neurite formation by modulating calcium dynamics |
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| المؤلفون: | Senís, E., Esgleas, M., Najas, S., Jiménez-Sábado, V., Bertani, C., Giménez-Alejandre, M., Escriche, A., Ruiz-Orera, J., Hergueta-Redondo, M., Jiménez, M., Giralt, A., Nuciforo, P., Albà, M.M., Peinado, H., Del Toro, D., Hove-Madsen, L., Götz, M., Abad, M. |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2021 |
| المجموعة: | Max-Delbrueck-Center for Molecular Medicine, Berlin: MDC Repository |
| مصطلحات موضوعية: | Cardiovascular and Metabolic Diseases, Topic 1: Genes, Cells and Cell-Based Medicine |
| الوصف: | Long noncoding RNAs (lncRNAs) are regulatory molecules which have been traditionally considered as "non-coding". Strikingly, recent evidence has demonstrated that many non-coding regions, including lncRNAs, do in fact contain small-open reading frames that code for small proteins that have been called microproteins. Only a few of them have been characterized so far, but they display key functions in a wide variety of cellular processes. Here, we show that TUNAR lncRNA encodes an evolutionarily conserved microprotein expressed in the nervous system that we have named pTUNAR. pTUNAR deficiency in mouse embryonic stem cells improves their differentiation potential towards neural lineage both in vitro and in vivo. Conversely, pTUNAR overexpression impairs neuronal differentiation by reduced neurite formation in different model systems. At the subcellular level, pTUNAR is a transmembrane protein that localizes in the endoplasmic reticulum and interacts with the calcium transporter SERCA2. pTUNAR overexpression reduces cytoplasmatic calcium, consistent with a possible role of pTUNAR as an activator of SERCA2. Altogether, our results suggest that our newly discovered microprotein has an important role in neural differentiation and neurite formation through the regulation of intracellular calcium. From a more general point of view, our results provide a proof of concept of the role of lncRNAs-encoded microproteins in neural differentiation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://edoc.mdc-berlin.de/21273/1/21273oa.pdfTest; http://edoc.mdc-berlin.de/21273/7/21273suppl.pdfTest; TUNAR lncRNA encodes a microprotein that regulates neural differentiation and neurite formation by modulating calcium dynamics. Senís, E. and Esgleas, M. and Najas, S. and Jiménez-Sábado, V. and Bertani, C. and Giménez-Alejandre, M. and Escriche, A. and Ruiz-Orera, J. and Hergueta-Redondo, M. and Jiménez, M. and Giralt, A. and Nuciforo, P. and Albà, M.M. and Peinado, H. and Del Toro, D. and Hove-Madsen, L. and Götz, M. and Abad, M. Frontiers in Cell and Developmental Biology 9 : 747667. 31 December 2021 |
| DOI: | 10.3389/fcell.2021.747667 |
| الإتاحة: | https://doi.org/10.3389/fcell.2021.747667Test http://edoc.mdc-berlin.de/21273Test/ https://edoc.mdc-berlin.de/21273Test/ http://edoc.mdc-berlin.de/21273/1/21273oa.pdfTest http://edoc.mdc-berlin.de/21273/7/21273suppl.pdfTest |
| حقوق: | cc_by_4 |
| رقم الانضمام: | edsbas.73919770 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fcell.2021.747667 |
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