دورية أكاديمية
Dermal fibroblasts are the key sensors of aseptic skin inflammation through interleukin 1 release by lesioned keratinocytes
| العنوان: | Dermal fibroblasts are the key sensors of aseptic skin inflammation through interleukin 1 release by lesioned keratinocytes |
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| المؤلفون: | Cordier-Dirikoc, Sevda, Pedretti, Nathalie, Garnier, Julien, Clarhaut-Charreau, Sandrine, Ryffel, Bernhard, Morel, Franck, Bernard, François-Xavier, Hamon de Almeida, Valérie, Lecron, Jean-Claude, Jégou, Jean-François |
| المساهمون: | QIMA Bioalternatives (QIMA Life Sciences), Laboratoire Inflammation, Tissus épithéliaux et Cytokines (LITEC Poitiers ), Université de Poitiers = University of Poitiers (UP), Immunologie et Neurogénétique Expérimentales et Moléculaires (INEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'immunologie CHU de Poitiers, Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie ) |
| المصدر: | ISSN: 1664-3224. |
| بيانات النشر: | HAL CCSD Frontiers |
| سنة النشر: | 2022 |
| المجموعة: | Université de Poitiers: Publications de nos chercheurs.ses (HAL) |
| مصطلحات موضوعية: | keratinocytes, fibroblasts, IL-1, skin, sterile inflammation, epidermal lesion, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology |
| الوصف: | International audience ; IL-1 plays a crucial role in triggering sterile inflammation following tissue injury. Although most studies associate IL-1 release by injured cells to the recruitment of neutrophils for tissue repair, the inflammatory cascade involves several molecular and cellular actors whose role remains to be specified. In the present study, we identified dermal fibroblasts among the IL-1R1-expressing skin cells as key sensors of IL-1 released by injured keratinocytes. After in vitro stimulation by recombinant cytokines or protein extracts of lysed keratinocytes containing high concentrations of IL-1, we show that dermal fibroblasts are by far the most IL-1-responsive cells compared to keratinocytes, melanocytes and endothelial cells. Fibroblasts have the property to respond to very low concentrations of IL-1 (from 10 fg/ml), even in the presence of 100-fold higher concentrations of IL-1RA, by increasing their expression of chemokines such as IL-8 for neutrophil recruitment. The capacity of IL-1-stimulated fibroblasts to attract neutrophils has been demonstrated both in vitro using cell migration assay and in vivo using a model of superficial epidermal lesion in IL-1R1-deficient mice which harbored reduced expression of inflammatory mediators and neutrophil skin infiltration. Together, our results shed a light on dermal fibroblasts as key relay cells in the chain of sterile inflammation induced after epidermal lesion. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03801039; https://hal.science/hal-03801039Test; https://hal.science/hal-03801039/documentTest; https://hal.science/hal-03801039/file/fimmu-13-984045.pdfTest |
| DOI: | 10.3389/fimmu.2022.984045 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2022.984045Test https://hal.science/hal-03801039Test https://hal.science/hal-03801039/documentTest https://hal.science/hal-03801039/file/fimmu-13-984045.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.737DE218 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2022.984045 |
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