كتاب
Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions
| العنوان: | Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions |
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| المؤلفون: | Trinkle-Mulcahy, Laura, Chusainow, Janet, Lam, Yun Wah, Swift, Sam, Lamond, Angus |
| المساهمون: | Moorhead, Greg |
| المصدر: | Trinkle-Mulcahy , L , Chusainow , J , Lam , Y W , Swift , S & Lamond , A 2007 , Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions . in G Moorhead (ed.) , Protein Phosphatase Protocols . Methods in Molecular Biology , vol. 365 , Springer , pp. 133-154 . https://doi.org/10.1385/1-59745-267-X:133Test |
| بيانات النشر: | Springer |
| سنة النشر: | 2007 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | FLIM, fluorescence, FRAP, FRET, GFP, isoforms, localization, microscopy, PP1, targeting, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics |
| الوصف: | Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division, signaling, differentiation, and metabolism. It is expressed in mammalian cells as three closely related isoforms: α, β/δ, and γ1. These isoforms differ in their relative affinities for proteins, termed targeting subunits , that mediate their intracellular localization and substrate specificity. Because of the dynamic nature of these interactions, it is important to find experimental approaches that permit direct analyses of PP1 localization and PP1-targeting subunit interactions in live cells. When transiently or stably expressed as fluorescent protein (FP) fusions, the three isoforms are active phosphatases with distinct localization patterns and can interact with both endogenous and exogenous targeting subunits. Their changing spatio-temporal distributions can be monitored both throughout the cell cycle and following cellular perturbations by time-lapse fluorescence microscopy, and turnover rates of intracellular pools of the protein calculated by fluorescence recovery after photobleaching (FRAP). Interactions with targeting subunits can be visualized in vivo by fluorescence resonance energy transfer (FRET), using techniques such as sensitized emission, acceptor photobleaching, or fluorescence lifetime imaging. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-1-58829-711-2 1-58829-711-X |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/5b3c1353-d255-46d3-8d98-f16525fee3a3Test; urn:ISBN:9781588297112 |
| DOI: | 10.1385/1-59745-267-X:133 |
| الإتاحة: | https://doi.org/10.1385/1-59745-267-X:133Test https://discovery.dundee.ac.uk/en/publications/5b3c1353-d255-46d3-8d98-f16525fee3a3Test http://www.scopus.com/inward/record.url?scp=34447273189&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.72611648 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781588297112 158829711X |
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| DOI: | 10.1385/1-59745-267-X:133 |