دورية أكاديمية
Interleukin-1β Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion
| العنوان: | Interleukin-1β Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion |
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| المؤلفون: | Monteleone, Mercedes, Stanley, Amanda, C, Chen, Kaiwen, W, Brown, Darren, L, Bezbradica, Jelena, S, von Pein, Jessica, B, Holley, Caroline, L, Boucher, Dave, Shakespear, Melanie, R, Kapetanovic, Ronan, Rolfes, Verena, Sweet, Matthew, J, Stow, Jennifer, L, Schroder, Kate |
| المساهمون: | Institute for Molecular Bioscience, The University of Queensland (UQ All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations ) |
| المصدر: | ISSN: 2211-1247. |
| بيانات النشر: | HAL CCSD Elsevier Inc |
| سنة النشر: | 2018 |
| المجموعة: | Institut National de la Recherche Agronomique: ProdINRA |
| مصطلحات موضوعية: | caspase-1, gasdermin, inflammasome, interleukin-1, macrophage, neutrophil, phosphoinositides, pyroptosis, trafficking, unconventional protein secretion, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; IL-1β requires processing by caspase-1 to generate the active, pro-inflammatory cytokine. Acute IL-1β secretion from inflammasome-activated macrophages requires caspase-1-dependent GSDMD cleavage, which also induces pyroptosis. Mechanisms of IL-1β secretion by pyroptotic and non-pyroptotic cells, and the precise functions of caspase-1 and GSDMD therein, are unresolved. Here, we show that, while efficient early secretion of endogenous IL-1β from primary non-pyroptotic myeloid cells in vitro requires GSDMD, later IL-1β release in vitro and in vivo proceeds independently of GSDMD. IL-1β maturation is sufficient for slow, caspase-1/GSDMD-independent secretion of ectopic IL-1β from resting, non-pyroptotic macrophages, but the speed of IL-1β release is boosted by inflammasome activation, via caspase-1 and GSDMD. IL-1β cleavage induces IL-1β enrichment at PIP2-enriched plasma membrane ruffles, and this is a prerequisite for IL-1β secretion and is mediated by a polybasic motif within the cytokine. We thus reveal a mechanism in which maturation-induced IL-1β trafficking facilitates its unconventional secretion. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-04346604; https://hal.inrae.fr/hal-04346604Test; https://hal.inrae.fr/hal-04346604/documentTest; https://hal.inrae.fr/hal-04346604/file/1-s2.0-S2211124718311173-main.pdfTest |
| DOI: | 10.1016/j.celrep.2018.07.027 |
| الإتاحة: | https://doi.org/10.1016/j.celrep.2018.07.027Test https://hal.inrae.fr/hal-04346604Test https://hal.inrae.fr/hal-04346604/documentTest https://hal.inrae.fr/hal-04346604/file/1-s2.0-S2211124718311173-main.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.7230AB3A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2018.07.027 |
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