تقرير
SH3 domains direct cellular localization of signaling molecules
| العنوان: | SH3 domains direct cellular localization of signaling molecules |
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| المؤلفون: | Bar-Sagi, D., Rotin, D., Batzer, A., Mandiyan, V., Schlessinger, J. |
| سنة النشر: | 1993 |
| المجموعة: | Cold Spring Harbor Laboratory: CSHL Institutional Repository |
| مصطلحات موضوعية: | phospholipase C, signal transduction |
| الوصف: | In this study we describe the cellular distribution of the SH2 and SH3 domains of phospholipase C-gamma (PLC-gamma) and of the adaptor protein GRB2 following their microinjection into living rat embryo fibroblasts. Using immunofluorescence microscopy, we show that a truncated protein composed of the SH2 and SH3 domains of PLC-gamma was localized to the actin cytoskeleton. A similar localization pattern was observed when only the SH3 domain of PLC-gamma was microinjected. In contrast, a truncated protein composed of only the SH2 domains of PLC-gamma exhibited diffuse cytoplasmic distribution. Microinjected GRB2 protein was localized primarily to membrane ruffles, as was GRB2 protein containing SH2 loss-of-function point mutations. Hence, the localization of GRB2 to membrane ruffles does not require interaction with tyrosine-phosphorylated moieties. However, GRB2 proteins with SH3 loss-of-function point mutations exhibited diffuse cytoplasmic distribution. These results indicate that SH3 domains are responsible for the targeting of signaling molecules to specific subcellular locations. |
| نوع الوثيقة: | report |
| اللغة: | unknown |
| العلاقة: | Bar-Sagi, D., Rotin, D., Batzer, A., Mandiyan, V., Schlessinger, J. (July 1993) SH3 domains direct cellular localization of signaling molecules. Cell, 74 (1). pp. 83-91. ISSN 0092-8674 (Print) |
| DOI: | 10.1016/0092-8674(93)90296-3 |
| الإتاحة: | https://doi.org/10.1016/0092-8674Test(93)90296-3 http://repository.cshl.edu/id/eprint/32490Test/ http://www.ncbi.nlm.nih.gov/pubmed/8334708Test |
| رقم الانضمام: | edsbas.720A0D81 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/0092-8674(93)90296-3 |
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