التفاصيل البيبلوغرافية
| العنوان: |
Midbody remnant inheritance Is regulated by the ESCRT subunit CHMP4C |
| المؤلفون: |
Casares-Arias, Javier, Ujue Gonzalez, María, San Paulo, Alvaro, Ventimiglia, Leandro N., Sadler, Jessica B.A., Miguez Gómez, David, Labat-de-Hoz, Leticia, Rubio-Ramos, Armando, Rangel, Laura, Bernabe-Rubio, Miguel, Fernandez-Barrera, Jaime, Correas Hornero, María Isabel, Martın-Serrano, Juan, Alonso, Miguel A. |
| المساهمون: |
UAM. Departamento de Física de la Materia Condensada |
| بيانات النشر: |
Cell Press |
| سنة النشر: |
2023 |
| المجموعة: |
Universidad Autónoma de Madrid (UAM): Biblos-e Archivo |
| مصطلحات موضوعية: |
Física |
| الوصف: |
The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage of ciliated cells. We demonstrate (1) that MBRs are physically connected to the plasma membrane, (2) how CHMP4C helps maintain the integrity of the connection, and (3) the functional importance of the connection ; The expert technical advice of the Optical and Confocal Microscopy Facility of CBMSO is gratefully acknowledged. We thank Dr. Phil Mason for revising the English language of the manuscript, Laura Fernandez-Martı´n for invaluable technical help, and David Esteban-Mendoza for his contribution to optimization of the SEM protocol. This work was supported by a grant (PGC2018-095643-B-I00) to M.A.A. from the Spanish Ministerio de Ciencia e Innovacion (MICIN), Agencia Estatal de Investigacion, y Fondo Europeo de Desarrollo Regional, European Union (MICIN/AEI/FEDER, UE), and by Wellcome Trust funding (WT102871MA) to J.M.-S. We also acknowledge the Micro and Nanofabrication Laboratory of the Instituto de Micro y Nanotecnologı´a (MiNa), which is funded by the Comunidad de Madrid (S2018/NMT-4291 TEC2-SPACE), MICIN (project CSIC13-4E-1794), and EU (FEDER, FSE), for invaluable help on SEM. A contract (FPU14/00295) and a short-term fellowship from EMBO to J.C.-A. are also acknowledged |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
iScience; https://doi.org/10.1016Test/ j.isci.2020.101244; Gobierno de España. PGC2018-095643-B-I00; Comunidad de Madrid. S2018/NMT-4291 TEC2-SPACE; iScience 23.6 (2020): 101244; 2589-0042 (online); http://hdl.handle.net/10486/708876Test; 101244-1; 101244-25; 23 |
| الإتاحة: |
http://hdl.handle.net/10486/708876Test |
| حقوق: |
© 2020 The Authors ; Reconocimiento – NoComercial – SinObraDerivada ; openAccess |
| رقم الانضمام: |
edsbas.71F9A5EC |
| قاعدة البيانات: |
BASE |
