دورية أكاديمية
The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code
| العنوان: | The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code |
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| المؤلفون: | Hofhuis, Julia, Schueren, Fabian, Nötzel, Christopher, Lingner, Thomas, Gärtner, Jutta, Jahn, Olaf, Thoms, Sven |
| سنة النشر: | 2016 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | Translational readthrough gives rise to C-terminally extended proteins, thereby providing the cell with new protein isoforms. These may have different properties from the parental proteins if the extensions contain functional domains. While for most genes amino acid incorporation at the stop codon is far lower than 0.1%, about 4% of malate dehydrogenase (MDH1) is physiologically extended by translational readthrough and the actual ratio of MDH1x (extended protein) to ‘normal' MDH1 is dependent on the cell type. In human cells, arginine and tryptophan are co-encoded by the MDH1x UGA stop codon. Readthrough is controlled by the 7-nucleotide high-readthrough stop codon context without contribution of the subsequent 50 nucleotides encoding the extension. All vertebrate MDH1x is directed to peroxisomes via a hidden peroxisomal targeting signal (PTS) in the readthrough extension, which is more highly conserved than the extension of lactate dehydrogenase B. The hidden PTS of non-mammalian MDH1x evolved to be more efficient than the PTS of mammalian MDH1x. These results provide insight into the genetic and functional co-evolution of these dually localized dehydrogenases. ; Open-Access-Publikationsfonds 2016 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2046-2441 |
| العلاقة: | SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz; SFB 1002 %7C A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz; SFB 1002 %7C S02: Hochauflösende Fluoreszenzmikroskopie und integrative Datenanalyse; RG Thoms (Biochemistry and Molecular Medicine); https://resolver.sub.uni-goettingen.de/purl?gro-2/13345Test; 3142222; https://sfb1002.med.uni-goettingen.de/production/literature/publications/276Test; https://resolver.sub.uni-goettingen.de/purl?gs-1/14098Test |
| DOI: | 10.1098/rsob.160246 |
| الإتاحة: | https://doi.org/10.1098/rsob.160246Test https://resolver.sub.uni-goettingen.de/purl?gro-2/13345Test https://resolver.sub.uni-goettingen.de/purl?gs-1/14098Test https://sfb1002.med.uni-goettingen.de/production/literature/publications/276Test |
| حقوق: | CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.71EFCDC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20462441 |
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| DOI: | 10.1098/rsob.160246 |