دورية أكاديمية
Microkeratome assisted deep lamellar keratoprosthesis
| العنوان: | Microkeratome assisted deep lamellar keratoprosthesis |
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| المؤلفون: | Shimmura, S, Miyashita, H, Uchino, Y, Taguchi, T, Kobayashi, H, Shimazaki, J, Tanaka, J, Tsubota, K |
| بيانات النشر: | British Medical Journal Publishing Group |
| سنة النشر: | 2006 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Clinical science - Scientific reports |
| الوصف: | Aims: To establish a keratoprosthesis (Kpro) surgical technique that maintains an intact superficial corneal layer. Methods: A manual microkeratome (Moria LSK-1) was used to create a 130 μm flap of approximately 10 mm diameter in the right eye of Japanese white rabbits. The stoma beneath the flap area was dissected before the removal of a 5.0 mm stromal disc. A 5.0 mm collagen I immobilised poly(vinyl alcohol) (COL-PVA) disc was placed on the exposed posterior stroma close to Descemet’s membrane. The flap was repositioned and fixed using 10-0 nylon sutures, which were removed 2 days following surgery. The corneas were followed clinically by slit lamp microscopy and photographs. Rabbits were sacrificed after 6 months, and the transplanted corneas were examined histologically by haematoxylin and eosin staining and immunohistochemistry against vimentin and α-smooth muscle actin (α-SMA). Results: The transplanted COL-PVA discs remained transparent throughout the study, with no complications related to the flap or overlying epithelium. The interface between COL-PVA and Descemet’s membrane remained clear without signs of opacification caused by scarring or cellular deposition. Pathology revealed the intact COL-PVA polymer in the posterior stroma, with minimal cellular infiltration along the anterior and posterior interfaces. Immunohistology shows vimentin and α-SMA staining at levels comparable to lamellar keratoplasty control. Conclusions: Microkeratome assisted deep lamellar keratoprosthesis may be a safe technique for the transplantation of artificial hydrogels for therapeutic purposes. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://bjo.bmj.com/cgi/content/short/90/7/826Test; http://dx.doi.org/10.1136/bjo.2006.090324Test |
| DOI: | 10.1136/bjo.2006.090324 |
| الإتاحة: | https://doi.org/10.1136/bjo.2006.090324Test http://bjo.bmj.com/cgi/content/short/90/7/826Test |
| حقوق: | Copyright (C) 2006, BMJ Publishing Group Ltd |
| رقم الانضمام: | edsbas.6FA233D6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/bjo.2006.090324 |
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