دورية أكاديمية
Rapid detection of vana/b-producing vancomycin-resistant enterococci using lateral flow immunoassay
| العنوان: | Rapid detection of vana/b-producing vancomycin-resistant enterococci using lateral flow immunoassay |
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| المؤلفون: | Oueslati, S., Gonzalez, C., Volland, H., Cattoir, V., Bernabeu, S., Girlich, D., Dulac, D., Plaisance, M., Boutigny, L., Dortet, L., Simon, S., Naas, T. |
| المساهمون: | Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes (IMVA-HB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de Pharmacologie et Immunoanalyse (SPI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Laboratoire d'Etudes et de Recherches en Immunoanalyses (LERI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS), Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Ponchaillou, ARN régulateurs bactériens et médecine (BRM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), NG Biotech, EIT Health, Agence Nationale de la Recherche, ANR: ANR-10-LABX-33, Institut National de la Santé et de la Recherche Médicale, Inserm: U1184, Université Paris-Saclay, ANR-10-LABX-0033,LERMIT,Research Laboratory on Drugs and Therapeutic Innovation(2010) |
| المصدر: | ISSN: 2075-4418 ; Diagnostics ; https://hal.science/hal-03420643Test ; Diagnostics, 2021, 11 (10), pp.1805. ⟨10.3390/diagnostics11101805⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | Immunochromatography, LFIA, Rapid diagnostics, Vancomycin resistance, VRE, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Vancomycin-resistant enterococci (VREs) have become one of the most important noso-comial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-char-acterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an effi-cient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already ex-isting tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34679500; hal-03420643; https://hal.science/hal-03420643Test; https://hal.science/hal-03420643/documentTest; https://hal.science/hal-03420643/file/diagnostics-11-01805-v2.pdfTest; PUBMED: 34679500; PUBMEDCENTRAL: PMC8534553; WOS: 000712850400001 |
| DOI: | 10.3390/diagnostics11101805 |
| الإتاحة: | https://doi.org/10.3390/diagnostics11101805Test https://hal.science/hal-03420643Test https://hal.science/hal-03420643/documentTest https://hal.science/hal-03420643/file/diagnostics-11-01805-v2.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6F9448AB |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/diagnostics11101805 |
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