التفاصيل البيبلوغرافية
| العنوان: |
Ancestral and homoplastic mutations in lldD2 augment Mtb fitness in lactate. |
| المؤلفون: |
Sydney Stanley, Xin Wang, Qingyun Liu, Young Yon Kwon, Abigail M. Frey, Nathan D. Hicks, Andrew J. Vickers, Sheng Hui, Sarah M. Fortune |
| سنة النشر: |
2024 |
| المجموعة: |
Smithsonian Institution: Figshare |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Genetics, Molecular Biology, Evolutionary Biology, Ecology, Cancer, Infectious Diseases, Computational Biology, Environmental Sciences not elsewhere classified, providing experimental evidence, div >< p, central carbon metabolism, 1 virulence system, mycobacterium tuberculosis <, discernible growth defect, dependent antibiotic susceptibility, mutations functionally increase, human host environment, global clinical isolates, lactate dehydrogenase reveals, assessing mtb genes, 13 , clinical isolates, vivo <, mtb growth, lactate dehydrogenase, intracellular environment, host pressure, genes involved |
| الوصف: |
(A) Growth curves of Mtb clinical strains from L1, L2, and L4. Strains are grouped for comparison of the lldD2 homoplastic mutation strains to closely related lineage ancestral strains. All cultures started at OD 600 0.005 at day 0. Triplicate replicates shown, error bars represent the standard deviation. (B) Quantification of the area under the curves (AUCs) from A. The AUC in 7H12–0.2% L-lactate is normalized to that of 7H9 for a given strain. The AUC is averaged for the V3I and V253M L4 strains and for the two L1 ancestral strains that were paired to -18 G>T. P-value indicates results of an unpaired t-test. (C) and (E) Schematic depicting the construction of recombinant lldD2 strains. (D) and (F) Growth curves of the strains indicated in (C) and (E). Hyg R , Kan R , and Zeo R refer to hygromycin, kanamycin, and zeocin resistance respectively. All cultures started at OD 600 0.005 at day 0. Triplicate replicates shown, error bars represent the standard deviation. Representative of two independent experiments. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/figure/Ancestral_and_homoplastic_mutations_in_i_lldD2_i_augment_Mtb_fitness_in_lactate_/25320180Test |
| DOI: |
10.1371/journal.ppat.1012050.g003 |
| الإتاحة: |
https://doi.org/10.1371/journal.ppat.1012050.g003Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.6F027F8E |
| قاعدة البيانات: |
BASE |
