دورية أكاديمية
siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition
| العنوان: | siRNA-Mediated MELK Knockdown Induces Accelerated Wound Healing with Increased Collagen Deposition |
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| المؤلفون: | Szymanski, Lukasz, Lewicki, Sławomir, Markiewicz, Tomasz, Cierniak, Szczepan, Tassan, Jean-Pierre, Kubiak, Jacek, Z. |
| المساهمون: | Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences (PAN), Kazimierz Pulaski University of Technology and Humanities in Radom, Military Institute of Medicine, Warsaw, Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), KOSCIUSZKO 1 No.571/2016/DA from the Polish Ministry of National Defense to J.Z.K. |
| المصدر: | ISSN: 1661-6596. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | MELK, fibroblasts, keratinocytes, mice, proliferation, rodents, siRNA, wound healing, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Skin wounds remain a significant problem for the healthcare system, affecting the clinical outcome, patients’ quality of life, and financial costs. Reduced wound healing times would improve clinical, economic, and social aspects for both patients and the healthcare system. Skin wound healing has been studied for years, but effective therapy that leads to accelerated wound healing remains to be discovered. This study aimed to evaluate the potential of MELK silencing to accelerate wound healing. A vectorless, transient knockdown of the MELK gene using siRNA was performed in a murine skin wound model. The wound size, total collagen, type 3 collagen, vessel size, vessel number, cell proliferation, cell apoptosis, number of mast cells, and immune infiltration by CD45, CD11b, CD45, and CD8a cells were evaluated. We observed that treatment with MELK siRNA leads to significantly faster wound closing associated with increased collagen deposition. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36674843; hal-03978836; https://hal.science/hal-03978836Test; https://hal.science/hal-03978836/documentTest; https://hal.science/hal-03978836/file/ijms-24-01326.pdfTest; PUBMED: 36674843 |
| DOI: | 10.3390/ijms24021326 |
| الإتاحة: | https://doi.org/10.3390/ijms24021326Test https://hal.science/hal-03978836Test https://hal.science/hal-03978836/documentTest https://hal.science/hal-03978836/file/ijms-24-01326.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6ED6B483 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms24021326 |
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