دورية أكاديمية
Comparative analysis of Stk11/Lkb1 versus Pten deficiency in lung adenocarcinoma induced by CRISPR/Cas9
| العنوان: | Comparative analysis of Stk11/Lkb1 versus Pten deficiency in lung adenocarcinoma induced by CRISPR/Cas9 |
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| المؤلفون: | Berthelsen, Martin F., Leknes, Siv L., Riedel, Maria, Pedersen, Mette A., Joseph, Justin V., Hager, Henrik, Vendelbo, Mikkel H., Thomsen, Martin K. |
| المصدر: | Berthelsen , M F , Leknes , S L , Riedel , M , Pedersen , M A , Joseph , J V , Hager , H , Vendelbo , M H & Thomsen , M K 2021 , ' Comparative analysis of Stk11/Lkb1 versus Pten deficiency in lung adenocarcinoma induced by CRISPR/Cas9 ' , Cancers , vol. 13 , no. 5 , 974 . https://doi.org/10.3390/cancers13050974Test |
| سنة النشر: | 2021 |
| المجموعة: | Aarhus University: Research |
| مصطلحات موضوعية: | Adenocarcinoma, CRISPR, Lung cancer, Mouse model, PTEN, STK11 |
| الوصف: | This study focused on STK11, PTEN, KRAS, and TP53, which are often found to be mu-tated in lung cancer. We compared Stk11 and Pten implication in lung cancer in combination with loss of Trp53 and gain of function of Kras in a CRISPR/Cas9 mouse model. Mice with loss of Stk11, Trp53, and KrasG12D mutation (SKT) reached human endpoint at around four months post-initia-tion. In comparison, mice with loss of Pten, Trp53, and KrasG12D mutation (PKT) survived six months or longer post-initiation. Pathological examination revealed an increase in proliferation in SKT deficient lung epithelia compared to PKT. This difference was independent of Pten loss, indi-cating that loss of Pten is dispensable for cell proliferation in lung adenocarcinoma. Furthermore, tumors with loss of Stk11, Trp53, and KrasG12D mutation had a significantly higher progression rate, monitored by PET/MRI scanning, compared to mice with loss of Pten, Trp53, and KrasG12D mutation, revealing that mutations in Stk11 are essential for adenocarcinoma progression. Overall, by using the CRISPR/Cas9 mouse model of lung adenocarcinoma, we showed that mutations in Stk11 are a key driver, whereas loss of Pten is dispensable for adenocarcinoma progression. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://pure.au.dk/portal/en/publications/879a46bc-347a-493b-b388-c64a846f769cTest |
| DOI: | 10.3390/cancers13050974 |
| الإتاحة: | https://doi.org/10.3390/cancers13050974Test https://pure.au.dk/portal/en/publications/879a46bc-347a-493b-b388-c64a846f769cTest http://www.scopus.com/inward/record.url?scp=85101533293&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6EC5B59F |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cancers13050974 |
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