دورية أكاديمية
Novel role for the midbody in primary ciliogenesis by polarized epithelial cells
| العنوان: | Novel role for the midbody in primary ciliogenesis by polarized epithelial cells |
|---|---|
| المؤلفون: | Bernabé-Rubio, Miguel, Andrés, Germán, Casares-Arias, Javier, Fernández-Barrera, Jaime, Rangel, Laura, Reglero Real, Natalia, Fernández, José J., Millán, Jaime, Correas Hornero, María Isabel, Miguez, David G., Alonso, Miguel A., Gershlick, David C. |
| بيانات النشر: | Rockefeller University Press |
| سنة النشر: | 2024 |
| المجموعة: | Universidad Autónoma de Madrid (UAM): Biblos-e Archivo |
| مصطلحات موضوعية: | Cell cycle and division, Cilia, Systems and Computational Biology, Biología y Biomedicina / Biología |
| الوصف: | The primary cilium is a membrane protrusion that is crucial for vertebrate tissue homeostasis and development. Here, we investigated the uncharacterized process of primary ciliogenesis in polarized epithelial cells. We show that after cytokinesis, the midbody is inherited by one of the daughter cells as a remnant that initially locates peripherally at the apical surface of one of the daughter cells. The remnant then moves along the apical surface and, once proximal to the centrosome at the center of the apical surface, enables cilium formation. The physical removal of the remnant greatly impairs ciliogenesis. We developed a probabilistic cell population-based model that reproduces the experimental data. In addition, our model explains, solely in terms of cell area constraints, the various observed transitions of the midbody, the beginning of ciliogenesis, and the accumulation of ciliated cells. Our findings reveal a biological mechanism that links the three microtubule-based organelles-the midbody, the centrosome, and the cilium-in the same cellular process ; This work was supported by the following grants from the Spanish Ministerio de Economía y Competitividad/Fondo Europeo de Desarrollo Regional: BFU2012-32532 and BFU2015-67266-R to M.A. Alonso, BFU2014-53299-P to D.G. Miguez, and TIN2012- 37483-C03-02 to J.F. Fernández. Grants from the Comunidad de Madrid (S2010/BMD-2305) to I. Correas and from Instituto de Investigaciones Sanitarias Jiménez Díaz to J. Millán are also acknowledged. M. Bernabé-Rubio and D.G. Miguez are the holders of a fellowship and a Ramón y Cajal contract (RYC-2010-07450), respectively, from the Ministerio de Economía y Competitividad. G. Andrés was supported by the Amarouto Program from the Comunidad de Madrid |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Journal of Cell Biology; https://doi.org/10.1083/jcb.201601020Test; Gobierno de España. BFU2012-32532; Gobierno de España. BFU2015-67266-R; Journal of Cell Biology 214.3 (2016): 259–273; 0021-9525 (print); 1540-8140 (online); http://hdl.handle.net/10486/710474Test; 259; 273; 214 |
| DOI: | 10.1083/jcb.201601020 |
| الإتاحة: | https://doi.org/10.1083/jcb.201601020Test http://hdl.handle.net/10486/710474Test |
| حقوق: | © 2016 Bernabé-Rubio et al ; Reconocimiento – NoComercial – CompartirIgual ; openAccess |
| رقم الانضمام: | edsbas.6E393CFB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.201601020 |
|---|