دورية أكاديمية
Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing
| العنوان: | Enzymatically amplified linear dbDNATM as a rapid and scalable solution to industrial lentiviral vector manufacturing |
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| المؤلفون: | Barreira, Maria, Kerridge, Claire, Jorda, Sara, Olofsson, Didrik, Neumann, Alexander, Horton, Helen, Smith-Moore, Sarah |
| المصدر: | Gene Therapy ; volume 30, issue 1-2, page 122-131 ; ISSN 0969-7128 1476-5462 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Genetics, Molecular Biology, Molecular Medicine |
| الوصف: | Traditional bacterial fermentation techniques used to manufacture plasmid are time-consuming, expensive, and inherently unstable. The production of sufficient GMP grade material thus imposes a major bottleneck on industrial-scale manufacturing of lentiviral vectors (LVV). Touchlight’s linear doggybone DNA (dbDNA TM ) is an enzymatically amplified DNA vector produced with exceptional speed through an in vitro dual enzyme process, enabling industrial-scale manufacturing of GMP material in a fraction of the time required for plasmid. We have previously shown that dbDNA TM can be used to produce functional LVV; however, obtaining high LVV titres remained a challenge. Here, we aimed to demonstrate that dbDNA TM could be optimised for the manufacture of high titre LVV. We found that dbDNA TM displayed a unique transfection and expression profile in the context of LVV production, which necessitated the optimisation of DNA input and construct ratios. Furthermore, we demonstrate that efficient 3’ end processing of viral genomic RNA (vgRNA) derived from linear dbDNA TM transfer vectors required the addition of a strong 3’ termination signal and downstream spacer sequence to enable efficient vgRNA packaging. Using these improved vector architectures along with optimised transfection conditions, we were able to produce a CAR19h28z LVV with equivalent infectious titres as achieved using plasmid, demonstrating that dbDNA TM technology can provide a highly effective solution to the plasmid bottleneck. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41434-022-00343-4 |
| الإتاحة: | https://doi.org/10.1038/s41434-022-00343-4Test https://www.nature.com/articles/s41434-022-00343-4.pdfTest https://www.nature.com/articles/s41434-022-00343-4Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.6D77E3F6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41434-022-00343-4 |
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