دورية أكاديمية
Evidence for serpinB2-independent protection from TNF-alpha-induced apoptosis
| العنوان: | Evidence for serpinB2-independent protection from TNF-alpha-induced apoptosis |
|---|---|
| المؤلفون: | Fish, Richard, Kruithof, Egbert |
| المصدر: | ISSN: 0014-4827 ; Experimental cell research, vol. 312, no. 3 (2006) p. 350-361. |
| سنة النشر: | 2006 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/616, Adaptor Proteins, Signal Transducing, Annexin A5/metabolism, Apoptosis/drug effects, Blotting, Western, Caspases/metabolism, Cell Proliferation/drug effects, Colonic Neoplasms/drug therapy/metabolism, Cycloheximide/pharmacology, DNA-Binding Proteins, Fibrosarcoma/drug therapy/metabolism, HeLa Cells, Humans, Intracellular Signaling Peptides and Proteins, Lentivirus/genetics, Mutagenesis, Site-Directed, Mutation/genetics, Nuclear Proteins, Plasminogen Activator Inhibitor 2/genetics/metabolism, Protein Synthesis Inhibitors/pharmacology, Proteins/metabolism, Receptors, Tumor Necrosis Factor, Type I/metabolism, Retinoblastoma Protein/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/metabolism |
| الوصف: | Clade B serine proteinase inhibitors (serpins) are intracellular proteins, whereas most of their identified targets are extracellular. A proposed intracellular role for these inhibitors is protection from apoptosis. We investigated the contribution of serpinB2 (plasminogen activator inhibitor-2, PAI-2) activity in TNF-alpha-induced apoptosis. PAI-2 is expressed in many normal and transformed cell types, particularly after stimulation with inflammatory cytokines. PAI-2 has been linked to protection from TNF-alpha-induced apoptosis, and a stabilizing interaction with the retinoblastoma protein (Rb1) has been proposed. We examined the activity of PAI-2 in TNF-alpha-induced apoptosis using HeLa, Isreco-1 and HT1080 cell lines. Stimulation with TNF-alpha protected each cell type from apoptosis induced by TNF-alpha and cycloheximide. Protection correlated with an increase in PAI-2 expression in IS-1 and HT1080 cells but not in HeLa cells where PAI-2 mRNA and protein were undetectable. PAI-2 was overexpressed in each cell type but gave no protection from TNF-alpha-induced apoptosis measured by cell viability, annexinV binding and caspase-3/7 activity. We detected wild-type Rb1, unchanged TNF receptor levels and induction of other apoptosis-protective factors in all cell types. In conclusion, elevated PAI-2 levels do not protect cells from TNF-alpha-induced apoptosis, and the protective effect of prior stimulation with TNF-alpha does not require PAI-2. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/16330024; https://archive-ouverte.unige.ch/unige:36191Test; unige:36191 |
| الإتاحة: | https://doi.org/10.1016/j.yexcr.2005.11.003Test https://archive-ouverte.unige.ch/unige:36191Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6D218EB3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |