دورية أكاديمية
Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors
| العنوان: | Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors |
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| المؤلفون: | Zhou, Fangmin, Tichy, Alexandra-Madelaine, Imambocus, Bibi Nusreen, Sakharwade, Shreyas, Rodriguez Jimenez, Francisco J., González Martínez, Marco, Jahan, Ishrat, Habib, Margarita, Wilhelmy, Nina, Burre, Vanessa, Lömker, Tatjana, Sauter, Kathrin, Helfrich-Förster, Charlotte, Pielage, Jan, Grunwald Kadow, Ilona C., Janovjak, Harald, Soba, Peter |
| المساهمون: | Deutsche Forschungsgemeinschaft, Chinese scholarship council |
| المصدر: | Nature Communications ; volume 14, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary |
| الوصف: | Neuromodulatory signaling via G protein-coupled receptors (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. The recent development of optogenetic tools to induce G protein-mediated signaling provides the promise of acute and cell type-specific manipulation of neuromodulatory signals. However, designing and deploying optogenetically functionalized GPCRs (optoXRs) with accurate specificity and activity to mimic endogenous signaling in vivo remains challenging. Here we optimize the design of optoXRs by considering evolutionary conserved GPCR-G protein interactions and demonstrate the feasibility of this approach using two Drosophila Dopamine receptors (optoDopRs). These optoDopRs exhibit high signaling specificity and light sensitivity in vitro. In vivo, we show receptor and cell type-specific effects of dopaminergic signaling in various behaviors, including the ability of optoDopRs to rescue the loss of the endogenous receptors. This work demonstrates that optoXRs can enable optical control of neuromodulatory receptor-specific signaling in functional and behavioral studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-023-43970-0 |
| الإتاحة: | https://doi.org/10.1038/s41467-023-43970-0Test https://www.nature.com/articles/s41467-023-43970-0.pdfTest https://www.nature.com/articles/s41467-023-43970-0Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.6C906EA4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-43970-0 |
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