التفاصيل البيبلوغرافية
| العنوان: |
Mechanical morphotype switching as an adaptive response in mycobacteria |
| المؤلفون: |
Eskandarian, Haig Alexander, Chen, Yu-Xiang, Toniolo, Chiara, Belardinelli, Juan M, Palcekova, Zuzana, Hom, Lesley, Ashby, Paul D, Fantner, Georg E, Jackson, Mary, McKinney, John D, Javid, Babak |
| المصدر: |
Science Advances, vol 10, iss 1 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2024 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Microbiology, Biological Sciences, Biomedical and Clinical Sciences, Infectious Diseases, Genetics, 2.2 Factors relating to the physical environment, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, Mycobacterium, Macrophages, Phagocytes, Cell Membrane |
| الوقت: |
eadh7957 |
| الوصف: |
Invading microbes face a myriad of cidal mechanisms of phagocytes that inflict physical damage to microbial structures. How intracellular bacterial pathogens adapt to these stresses is not fully understood. Here, we report the discovery of a virulence mechanism by which changes to the mechanical stiffness of the mycobacterial cell surface confer refraction to killing during infection. Long-term time-lapse atomic force microscopy was used to reveal a process of "mechanical morphotype switching" in mycobacteria exposed to host intracellular stress. A "soft" mechanical morphotype switch enhances tolerance to intracellular macrophage stress, including cathelicidin. Both pharmacologic treatment, with bedaquiline, and a genetic mutant lacking uvrA modified the basal mechanical state of mycobacteria into a soft mechanical morphotype, enhancing survival in macrophages. Our study proposes microbial cell mechanical adaptation as a critical axis for surviving host-mediated stressors. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt3903z7dr; https://escholarship.org/uc/item/3903z7drTest |
| الإتاحة: |
https://escholarship.org/uc/item/3903z7drTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.6C881A59 |
| قاعدة البيانات: |
BASE |
