دورية أكاديمية
Colorectal carcinoma peritoneal metastases-derived organoids: results and perspective of a model for tailoring hyperthermic intraperitoneal chemotherapy from bench-to-bedside ...
| العنوان: | Colorectal carcinoma peritoneal metastases-derived organoids: results and perspective of a model for tailoring hyperthermic intraperitoneal chemotherapy from bench-to-bedside ... |
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| المؤلفون: | Varinelli, Luca, Battistessa, Davide, Guaglio, Marcello, Zanutto, Susanna, Illescas, Oscar, Lorenc, Ewelina J., Pisati, Federica, Kusamura, Shigeki, Cattaneo, Laura, Sabella, Giovanna, Milione, Massimo, Perbellini, Alessia, Noci, Sara, Paolino, Cinzia, Khun, Elisabetta, Galassi, Margherita, Cavalleri, Tommaso, Deraco, Marcello, Gariboldi, Manuela, Baratti, Dario |
| بيانات النشر: | figshare |
| سنة النشر: | 2024 |
| المجموعة: | DataCite Metadata Store (German National Library of Science and Technology) |
| مصطلحات موضوعية: | Biochemistry, Medicine, Cell Biology, Pharmacology, Biotechnology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Cancer, Science Policy |
| الوصف: | Background Peritoneal metastases from colorectal cancer (CRCPM) are related to poor prognosis. Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) have been reported to improve survival, but peritoneal recurrence rates are still high and there is no consensus on the drug of choice for HIPEC. The aim of this study was to use patient derived organoids (PDO) to build a relevant CRCPM model to improve HIPEC efficacy in a comprehensive bench-to-bedside strategy. Methods Oxaliplatin (L-OHP), cisplatin (CDDP), mitomycin-c (MMC) and doxorubicin (DOX) were used to mimic HIPEC on twelve PDO lines derived from twelve CRCPM patients, using clinically relevant concentrations. After chemotherapeutic interventions, cell viability was assessed with a luminescent assay, and the obtained dose–response curves were used to determine the half-maximal inhibitory concentrations. Also, induction of apoptosis by different HIPEC interventions on PDOs was studied by evaluating CASPASE3 cleavage. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.6084/m9.figshare.c.7215753 |
| الإتاحة: | https://doi.org/10.6084/m9.figshare.c.7215753Test https://springernature.figshare.com/collections/Colorectal_carcinoma_peritoneal_metastases-derived_organoids_results_and_perspective_of_a_model_for_tailoring_hyperthermic_intraperitoneal_chemotherapy_from_bench-to-bedside/7215753Test |
| حقوق: | Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcodeTest ; cc-by-4.0 |
| رقم الانضمام: | edsbas.6BEF70F4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.6084/m9.figshare.c.7215753 |
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