دورية أكاديمية
RT-QuIC Detection of Pathological α-Synuclein in Skin Punches of Patients with Lewy Body Disease
| العنوان: | RT-QuIC Detection of Pathological α-Synuclein in Skin Punches of Patients with Lewy Body Disease |
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| المؤلفون: | Mammana A., Baiardi S., Rossi M., Donadio V., Capellari S., Liguori R., Parchi P., Quadalti C. |
| المساهمون: | Mammana A., Baiardi S., Quadalti C., Rossi M., Donadio V., Capellari S., Liguori R., Parchi P. |
| سنة النشر: | 2021 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | biomarker, CSF, prion, real-time quaking-induced conversion, synucleinopathie, Autopsy, Human, Skin, Lewy Body Disease, alpha-Synuclein |
| الوصف: | Background: Evidence suggests that skin represents a suitable matrix for demonstrating α-synuclein oligomers as a diagnostic biomarker for Lewy body disease. Objective: The objective of this study was to evaluate the diagnostic performance of skin α-syn real-time quaking-induced conversion assay in patients with Lewy body disease. Methods: We analyzed skin punches taken in vitam (n=69) or postmortem (n=49) from patients with PD, dementia with Lew bodies (DLB), incidental Lewy body pathology, and neurological controls. Seventy-nine patients underwent both CSF and skin α-synuclein real-time quaking-induced conversion assay. Results: Overall, the skin α-synuclein real-time quaking-induced conversion assay distinguished Lewy body disease patients with 94.1% accuracy (sensitivity, 89.2%; specificity, 96.3%). Assay sensitivity reached 94.1% in the 17 Lewy body disease patients analyzed in the cervical region. In patients with both CSF and skin samples, the 2 real-time quaking-induced conversion assay protocols yielded similar diagnostic accuracy (skin, 97.5%; CSF, 98.7%). Conclusion: Skin punch biopsies might represent a valid and convenient alternative to CSF analysis to demonstrate Lew body-related α-synuclein deposition in patients with Lewy body disease. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34002890; info:eu-repo/semantics/altIdentifier/wos/WOS:000651481400001; volume:36; issue:9; firstpage:2173; lastpage:2177; numberofpages:5; journal:MOVEMENT DISORDERS; https://hdl.handle.net/11585/851921Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85105866499 |
| DOI: | 10.1002/mds.28651 |
| الإتاحة: | https://doi.org/10.1002/mds.28651Test https://hdl.handle.net/11585/851921Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6BE8AF4F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/mds.28651 |
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