دورية أكاديمية
Identification of 3'-terminal exons from yeast artificial chromosomes.
| العنوان: | Identification of 3'-terminal exons from yeast artificial chromosomes. |
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| المؤلفون: | Krizman, D B, Hofmann, T A, DeSilva, U, Green, E D, Meltzer, P S, Trent, J M |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 1995 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | ARTICLES |
| الوصف: | We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://genome.cshlp.org/cgi/content/short/4/6/322Test; http://dx.doi.org/10.1101/gr.4.6.322Test |
| DOI: | 10.1101/gr.4.6.322 |
| الإتاحة: | https://doi.org/10.1101/gr.4.6.322Test http://genome.cshlp.org/cgi/content/short/4/6/322Test |
| حقوق: | Copyright (C) 1995, Cold Spring Harbor Laboratory Press |
| رقم الانضمام: | edsbas.6B5B1338 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/gr.4.6.322 |
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