دورية أكاديمية
Global methylome scores correlate with histological subtypes of colorectal carcinoma and show different associations with common clinical and molecular features
| العنوان: | Global methylome scores correlate with histological subtypes of colorectal carcinoma and show different associations with common clinical and molecular features |
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| المؤلفون: | Turpín‐Sevilla, M.D.C., Pérez‐Sanz, F., García‐Solano, J., Sebastián‐León, P., Trujillo‐Santos, J., Carbonell, P., Estrada Alonso, Eduardo, Rodríguez‐Braun, E., Conesa, A., Conesa‐Zamora, P., Tuomisto, A., Herruzo, I., Fennell, L.J., Makinen, M.J., Whitehall, V.L.J. |
| المساهمون: | UAM. Departamento de Psicología Social y Metodología |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Universidad Autónoma de Madrid (UAM): Biblos-e Archivo |
| مصطلحات موضوعية: | colorectal carcinoma, serrated pathway, microsatellite instability, serrated adenocarcinoma, CIMP, DNA methylation, epigenetics of carcinogenesis, methylome, conventional carcinoma, methylation score, Psicología |
| الوصف: | Background. The typical methylation patterns associated with cancer are hypermethylation at gene promoters and global genome hypomethylation. Aberrant CpG island hypermethylation at promoter regions and global genome hypomethylation have not been associated with histological colorectal carcinomas (CRC) subsets. Using Illumina’s 450 k Infinium Human Methylation beadchip, the methylome of 82 CRCs were analyzed, comprising different histological subtypes: 40 serrated adenocarcinomas (SAC), 32 conventional carcinomas (CC) and 10 CRCs showing histological and molecular features of microsatellite instability (hmMSI‐H), and, additionally, 35 normal adjacent mucosae. Scores reflecting the overall methylation at 250 bp, 1 kb and 2 kb from the transcription starting site (TSS) were studied. Results. SAC has an intermediate methylation pattern between CC and hmMSI‐H for the three genome locations. In addition, the shift from promoter hypermethylation to genomic hypomethylation occurs at a small sequence between 250 bp and 1 Kb from the gene TSS, and an asymmetric distribution of methylation was observed between both sides of the CpG islands (N vs. S shores). Conclusion. These findings show that different histological subtypes of CRC have a particular global methylation pattern depending on sequence distance to TSS and highlight the so far underestimated importance of CpGs aberrantly hypomethylated in the clinical phenotype of CRCs. ; This work was supported by two grants from Instituto de Salud Carlos III, Ministerio de Sanidad, Spain and FEDER funds (refs: PI12-1232, PI18-0144) and another from the European Union’s Horizon 2020 research and innovation program (ref. 848098) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6694 |
| العلاقة: | Cancers; https://doi.org/10.3390/cancers13205165Test; info:eu-repo/grantAgreement/EC/H2020/ 848098; Gobierno de España. PI12-1232; Gobierno de España. PI18-0144; Cancers 13.20 (2021): 5165; http://hdl.handle.net/10486/706283Test; 5165; 20; 13 |
| الإتاحة: | https://doi.org/10.3390/cancers13205165Test http://hdl.handle.net/10486/706283Test |
| حقوق: | © 2021 by the authors ; Reconocimiento ; openAccess |
| رقم الانضمام: | edsbas.6B43BF49 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
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