دورية أكاديمية
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
| العنوان: | Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins |
|---|---|
| المؤلفون: | Chauvin, Brieuc, Nakazawa, Koyomi, Beber, Alexandre, Di Cicco, Aurélie, Hajj, Bassam, Iv, François, Mavrakis, Manos, Koenderink, Gijsje, Cabral, João, Trichet, Michaël, Mangenot, Stéphanie, Bertin, Aurélie |
| المساهمون: | Laboratoire Physico-Chimie Curie Institut Curie (PCC), Institut Curie Paris -Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institute of Biotechnology of the Czech Academy of Sciences (IBT / CAS), Czech Academy of Sciences Prague (CAS), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Kavli Institute of Nanosciences Delft (KI-NANO), Delft University of Technology (TU Delft), Department of Chemical Engineering London, Imperial College London, Microscopie Electronique IBPS (IBPS-ME), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), B. Chauvin is funded by the Ecole Doctorale "ED564: Physique en Ile de France" and Fondation pour lea Recherche Médicale. K. Nakazawa was supported by Sorbonne Université (AAP Emergence). G.H. Koenderink was supported by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO/OCW) through the ‘BaSyC-Building a Synthetic Cell'. Gravitation grant (024.003.019)., ANR-13-JSV8-0002,SEPTIME,Assemblage et ultrastructure des septines, de la molécule à la cellule(2013), ANR-17-CE13-0014,SEPTIMORF,Rôle des septines dans la morphogenèse des cellules animales(2017), ANR-20-CE11-0014,SeptScort,Interaction spatiale et fonctionnelle entre septines et Escrt pendant la cytocinèse: une approche multi-échelle(2020), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-11-LABX-0038,CelTisPhyBio,Des cellules aux tissus: au croisement de la Physique et de la Biologie(2011) |
| المصدر: | ISSN: 1940-087X. |
| بيانات النشر: | HAL CCSD JoVE |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect the role of the environment (ionic conditions, protein and lipid compositions, membrane curvature) and the different partners associated with specific membrane reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest in revealing the role of septin proteins associated with major diseases. Septins are essential and ubiquitous cytoskeletal proteins that interact with the plasma membrane. They are implicated in cell division, cell motility, neuro-morphogenesis, and spermiogenesis, among other functions. It is, therefore, important to understand how septins interact and organize at membranes to subsequently induce membrane deformations and how they can be sensitive to specific membrane curvatures. This article aims to decipher the interplay between the ultra-structure of septins at a molecular level and the membrane remodeling occurring at a micron scale. To this end, budding yeast, and mammalian septin complexes were recombinantly expressed and purified. A combination of in vitro assays was then used to analyze the self-assembly of septins at the membrane. Supported lipid bilayers (SLBs), giant unilamellar vesicles (GUVs), large unilamellar vesicles (LUVs), and wavy substrates were used to study the interplay between septin self-assembly, membrane reshaping, and membrane curvature. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-04237668; https://hal.science/hal-04237668Test; https://hal.science/hal-04237668/documentTest; https://hal.science/hal-04237668/file/jove.pdfTest |
| DOI: | 10.3791/63889 |
| الإتاحة: | https://doi.org/10.3791/63889Test https://hal.science/hal-04237668Test https://hal.science/hal-04237668/documentTest https://hal.science/hal-04237668/file/jove.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6B422303 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3791/63889 |
|---|