تقرير
Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum
| العنوان: | Metabolic and Process Engineering for Microbial Production of Protocatechuate from Xylose with Corynebacterium glutamicum |
|---|---|
| المؤلفون: | Labib, Mohamed, Görtz, Jonas, Brüsseler, Christian, Kallscheuer, Nicolai, Gätgens, Jochem, Jupke, Andreas, Marienhagen, Jan, Noack, Stephan |
| المصدر: | doi:10.1101/2021.02.12.430943 |
| سنة النشر: | 2021 |
| المجموعة: | Forschungszentrum Jülich: JuSER (Juelich Shared Electronic Resources) |
| جغرافية الموضوع: | DE |
| الوصف: | 3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA is of interest in the chemical industry as a building block for novel polymers and has wide potential for pharmaceutical applications due to its antioxidant, anti-inflammatory, and antiviral properties. In the present study, we designed and constructed a novel Corynebacterium glutamicum strain to enable the efficient utilization of d -xylose for microbial production of PCA. The engineered strain showed a maximum PCA titer of 62.1 ± 12.1 mM (9.6 ± 1.9 g L −1 ) from d -xylose as the primary carbon and energy source. The corresponding yield was , which corresponds to 38 % of the maximum theoretical yield and is 14-fold higher compared to the parental producer strain on d -glucose. By establishing a one-pot bioreactor cultivation process followed by subsequent process optimization, the same maximum titer and a total amount of 16.5 ± 1.1 g was reached. Downstream processing of PCA from this fermentation broth was realized via electrochemically induced crystallization by taking advantage of the pH-dependent properties of PCA. Since PCA turned out to be electrochemically unstable in combination with several anode materials, a threechamber electrolysis setup was established to crystallize PCA and to avoid direct anode contact. This resulted in a maximum final purity of 95.4 %. In summary, the established PCA production process represents a highly sustainable approach, which will serve as a blueprint for the bio-based production of other hydroxybenzoic acids from alternative sugar feedstocks. |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/hdl/2128/28599; https://juser.fz-juelich.de/record/894762Test; https://juser.fz-juelich.de/search?p=id:%22FZJ-2021-03376%22Test |
| الإتاحة: | https://doi.org/10.1101/2021.02.12.430943Test https://juser.fz-juelich.de/record/894762Test https://juser.fz-juelich.de/search?p=id:%22FZJ-2021-03376%22Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6AD96881 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |