دورية أكاديمية
Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller
| العنوان: | Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller |
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| المؤلفون: | Lorin, Valerie, Fernández, Ignacio, Masse-Ranson, Guillemette, Bouvin-Pley, Mélanie, Molinos-Albert, Luis, M., Planchais, Cyril, Hieu, Thierry, Péhau-Arnaudet, Gérard, Hrebík, Dominik, Girelli-Zubani, Giulia, Fiquet, Oriane, Guivel-Benhassine, Florence, Sanders, Rogier, W., Walker, Bruce, D., Schwartz, Olivier, Scheid, Johannes, F., Dimitrov, Jordan, D., Plevka, Pavel, Braibant, Martine, Seaman, Michael, S., Bontems, François, Di Santo, James, P., Rey, Félix, A., Mouquet, Hugo |
| المساهمون: | Immunologie humorale - Humoral Immunology, Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Virologie Structurale, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Immunité Innée - Innate Immunity, Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents (MAVIVHe), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagopole (CITECH), Institut Pasteur Paris (IP), Microscopie Ultrastructurale (Plate-forme), Masaryk University Brno (MUNI), Virus et Immunité - Virus and immunity (CNRS-UMR3569), University of Amsterdam Amsterdam = Universiteit van Amsterdam (UvA), Weill Medical College of Cornell University New York, Massachusetts General Hospital Boston, Harvard Medical School Boston (HMS), Rockefeller University New York, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Beth Israel Deaconess Medical Center Boston (BIDMC), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), The NanoImaging Core was created with the help of a grant from the French Government’s Investissements d’Avenir program (EQUIPEX CACSICE - Centre d’analyse de systèmes complexes dans les environnements complexes, ANR-11-EQPX-0008). We thank the NIH AIDS Reagent Program (Division of AIDS, National Institute of Allergy and Infectious Diseases, NIH) for contributing to reagents and the Agence National de Recherche sur le SIDA et les hépatites virales (ANRS) for an equipment grant support. The Biomics platform is supported by France Génomique (ANR-10-INBS-09-09) and IBISA. I. Fernández and C. Planchais were recipients of an ANRS postdoctoral fellowship. The J.P. Di Santo laboratory received support from the Vaccine Research Institute (Créteil, France). H. Mouquet received core grants from the Institut Pasteur, Institut national de la santé et de la recherche médicale, and the Milieu Intérieur Program (ANR-10-LABX-69-01). This work was supported by the European Research Council Seventh Framework Program (ERC-2013-StG 337146), and by Gilead Sciences HIV Cure Grants (#00397)., ANR-11-EQPX-0008,CACSICE,Centre d'analyse de systèmes complexes dans les environnements complexes(2011), ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-10-LABX-0069,MILIEU INTERIEUR,GENETIC & ENVIRONMENTAL CONTROL OF IMMUNE PHENOTYPE VARIANCE: ESTABLISHING A PATH TOWARDS PERSONALIZED MEDICINE(2010), European Project: 337146,EC:FP7:ERC,ERC-2013-StG,HUMANTIVIRUSES(2014) |
| المصدر: | ISSN: 0022-1007. |
| بيانات النشر: | HAL CCSD Rockefeller University Press |
| سنة النشر: | 2022 |
| المجموعة: | Université François-Rabelais de Tours: HAL |
| مصطلحات موضوعية: | [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | International audience ; Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs of three distinct B cell lineages in a viremic controller, two of which comprised only IgG+ or IgA+ blood memory B cells; the third combined both IgG and IgA clonal variants. 7-269 bNAb in the IgA-only lineage displayed the highest neutralizing capacity despite limited somatic mutation, and delayed viral rebound in humanized mice. bNAbs in all three lineages targeted the N332 glycan supersite. The 2.8-Å resolution cryo-EM structure of 7-269-BG505 SOSIP.664 complex showed a similar pose as 2G12, on an epitope mainly composed of sugar residues comprising the N332 and N295 glycans. Binding and cryo-EM structural analyses showed that antibodies from the two other lineages interact mostly with glycans N332 and N386. Hence, multiple B cell lineages of IgG and IgA bNAbs focused on a unique HIV-1 site of vulnerability can codevelop in HIV-1 viremic controllers. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/35230385; info:eu-repo/grantAgreement/EC/FP7/337146/EU/Human Antibody Responses to Viruses/HUMANTIVIRUSES; pasteur-03654254; https://pasteur.hal.science/pasteur-03654254Test; https://pasteur.hal.science/pasteur-03654254/documentTest; https://pasteur.hal.science/pasteur-03654254/file/jem_20212045.pdfTest; PUBMED: 35230385; PUBMEDCENTRAL: PMC8932546 |
| DOI: | 10.1084/jem.20212045 |
| الإتاحة: | https://doi.org/10.1084/jem.20212045Test https://pasteur.hal.science/pasteur-03654254Test https://pasteur.hal.science/pasteur-03654254/documentTest https://pasteur.hal.science/pasteur-03654254/file/jem_20212045.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-nc-saTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6A735EB4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1084/jem.20212045 |
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