دورية أكاديمية
Enterotoxin- and Antibiotic-Resistance-Encoding Genes Are Present in Both Coagulase-Positive and Coagulase-Negative Foodborne Staphylococcus Strains
| العنوان: | Enterotoxin- and Antibiotic-Resistance-Encoding Genes Are Present in Both Coagulase-Positive and Coagulase-Negative Foodborne Staphylococcus Strains |
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| المؤلفون: | Salamandane, Acácio, Oliveira, Jessica, Coelho, Miguel, Ramos, Beatriz, Cunha, Mónica V., Malfeito-Ferreira, Manuel, Brito, Luisa |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Universidade de Lisboa: repositório.UL |
| الوصف: | Food poisoning by staphylococcal enterotoxins (SE) is a major cause of foodborne illness, often associated with coagulase-positive staphylococci (CPS). The increase in the number of methicillin-resistant Staphylococcus aureus (MRSA) strains is another major problem associated with CPS. However, reports of the association of SE and methicillin-resistant Staphylococcus with coagulase-negative staphylococci (CNS) are beginning to re-emerge. In this context, the aim of this study is to investigate the presence of staphylococcal enterotoxin genes and to characterize the phenotypic and genotypic antimicrobial resistance in 66 isolates of Staphylococcus spp. (47 CNS and 19 CPS) recovered from ready-to-eat (RTE) street food sold in Maputo, Mozambique. Seven virulence genes encoding SE (sea, seb, sec, sed and see) and two toxins (hlb and sak) were screened by multiplex PCR (MPCR). Antimicrobial resistance against 12 antibiotics was evaluated by the disk diffusion method. The presence of genes encoding resistance to penicillin, methicillin, vancomycin and erythromycin (blaZ, mecA, vancA, vancB, ermA, ermB and ermC) were also screened by PCR. At least one of the seven virulence genes assessed in this study was detected in 57.9% and 51% of CPS and CNS isolates, respectively. In CPS isolates, the most frequent gene was hlb (47.4%), followed by sec (15.8%) and sea, seb and sed genes with 5.3% each. In CNS isolates, the most frequent gene was sec (36.2%) followed by sak (17%), hlb (14.9%), sed (12.8%) and seb (6.4%). Five of the twelve CPS in which virulence genes were detected were also antibiotic-resistant. All the CNS isolates harboring virulence genes (n = 27, 57.4%) were antimicrobial-resistant. The prevalence of multidrug resistance was higher (59.6%) in CNS than in CPS (26.3%) isolates. Regarding the presence of antibiotic-resistance genes, blaZ (penicillin-resistant) was the most frequent in both CPS (42.1%) and CNS (87.2%), followed by the mecA (encoding methicillin resistance) and vancA genes (vancomycin-resistant), ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | FCT PRT/BD/151521/2021, DFA/BD/7777/2020,UIDB/04129/2020, UIDB/00329/2020, UIDB/04046/2020, LA/P/0121/2020; Salamandane, A.; Oliveira, J.; Coelho, M.; Ramos, B.; Cunha, M.V.; Malfeito-Ferreira, M.; Brito, L. Enterotoxin- and Antibiotic-Resistance-Encoding Genes Are Present in Both Coagulase-Positive and Coagulase-Negative Foodborne Staphylococcus Strains. Appl. Microbiol. 2022, 2, 367-380. https://doi.org/10.3390/applmicrobiol2020028Test; http://hdl.handle.net/10451/55919Test |
| DOI: | 10.3390/applmicrobiol2020028 |
| الإتاحة: | https://doi.org/10.3390/applmicrobiol2020028Test http://hdl.handle.net/10451/55919Test |
| حقوق: | openAccess ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.690949C8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/applmicrobiol2020028 |
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