دورية أكاديمية
Natriuretic peptide receptors in the central vasculature of the toad, Bufo marinus
| العنوان: | Natriuretic peptide receptors in the central vasculature of the toad, Bufo marinus |
|---|---|
| المؤلفون: | K Minerds, John Donald |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Animal physiology - biophysics, Animal physiology - cell, Animal physiology - systems, Zoology not elsewhere classified, atrial natriuretic peptide, blood vessels, clearance receptors, guanylyl cyclase, cGMP, natriuretic peptide receptors, c-type natriuretic peptide, Bufo marinus, 060603 Animal Physiology - Systems |
| الوصف: | Natriuretic peptide receptors in the central vasculature of the toad, Bufo marinus , were characterized using autoradiographical, molecular, and physiological techniques. Specific 125 I-rat ANP binding sites were present in the carotid and pulmonary arteries, the lateral aorta, the pre- and post-cava, and the jugular vein, and generally occurred in each layer of the blood vessel. The 12 5I-rat ANP binding was partially displaced by the specific natriuretic peptide receptor C ligand, C-ANF, which indicates the presence of two types of natriuretic peptide receptors in the blood vessels. This was confirmed by a RT-PCR study, which demonstrated that guanylyl cyclase receptor (NPR-GC) and NPR-C mRNAs are expressed in arteries and veins. An in vitro guanylyl cyclase assay showed that frog ANP stimulated the production of cGMP in arterial membrane fractions. Physiological recordings from isolated segments of the carotid and pulmonary arteries and the lateral aorta, which had been pre-constricted with arginine vasotocin, showed that rat ANP, frog ANP and porcine CNP relaxed the vascular smooth muscle with relatively similar potency. Together, the data show that the central vasculature contains two types of natriuretic peptide receptors (NPR-C and NPR-GC) and that the vasculature is a target for ANP and CNP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/10536/DRO/DU:30001284Test; https://figshare.com/articles/journal_contribution/Natriuretic_peptide_receptors_in_the_central_vasculature_of_the_toad_Bufo_marinus/20529981Test |
| الإتاحة: | http://hdl.handle.net/10536/DRO/DU:30001284Test https://figshare.com/articles/journal_contribution/Natriuretic_peptide_receptors_in_the_central_vasculature_of_the_toad_Bufo_marinus/20529981Test |
| حقوق: | All Rights Reserved |
| رقم الانضمام: | edsbas.68F83839 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |