دورية أكاديمية
Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome
| العنوان: | Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome |
|---|---|
| المؤلفون: | González-Tablas, María, Otero, Álvaro, Arandia, Daniel, Pascual-Argente, Daniel, Ruiz-Martin, Laura, Sousa, Pablo, García-Martin, Andoni, Roa Montes de Oca, Juan Carlos, Villaseñor-Ledezma, Javier, Carretero, Luis Torres, Almeida, María, Ortiz, Javier, Nieto, Adelaida, Orfao, Alberto, Tabernero, María D. |
| المساهمون: | Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), European Commission, Junta de Castilla y León |
| بيانات النشر: | John Wiley & Sons |
| سنة النشر: | 2021 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Glioblastoma, Immune cells, Lymphocytes, Microenvironment, Microglia, Myeloid cells |
| الوصف: | © 2020 The Authors. ; The distribution and role of tumor-infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition of 55 primary (adult) GBM samples by flow cytometry and correlated the tumor immune profile with patient features at diagnosis and outcome. GBM single-cell suspensions were stained at diagnosis (n = 44) and recurrence following radiotherapy and chemotherapy (n = 11) with a panel of 8-color monoclonal antibody combinations for the identification and enumeration of (GFAPCD45) tumor and normal astrocytic cells, infiltrating myeloid cells —i.e. microglial and blood-derived tumor-associated macrophages (TAM), M1-like, and M2-like TAM, neutrophils. and myeloid-derived suppressor cells (MDSC)— and tumor-infiltrating lymphocytes (TIL) —i.e. CD3T-cells and their TCD4, TCD8, TCD4CD8, and (CD25CD127) regulatory (T-regs) subsets, (CD19CD20) B-cells, and (CD16) NK-cells—. Overall, GBM samples consisted of a major population (mean ± 1SD) of tumor and normal astrocytic cells (73% ± 16%) together with a significant but variable fraction of immune cells (24% ± 18%). Within myeloid cells, TAM predominated (13% ± 12%) including both microglial cells (10% ± 11%) and blood-derived macrophages (3% ± 5%), in addition to a smaller proportion of neutrophils (5% ± 9%) and MDSC (4% ± 8%). Lymphocytes were less represented and mostly included TCD4 (0.5% ± 0.7%) and TCD8 cells (0.6% ± 0.7%), together with lower numbers of TCD4CD8 T-cells (0.2% ± 0.4%), T-regs (0.1% ± 0.2%), B-lymphocytes (0.1% ± 0.2%) and NK-cells (0.05% ± 0.05%). Overall, three distinct immune profiles were identified: cases with a minor fraction of leucocytes, tumors with a predominance of TAM and neutrophils, and cases with mixed infiltration by TAM, neutrophils, and T-lymphocytes. Untreated GBM patients with mixed myeloid and lymphoid immune infiltrates showed a significantly shorter patient overall survival versus the other two groups, in the absence of gains of the EGFR gene (p = 0.02). Here we ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1015-6305 1750-3639 |
| العلاقة: | Publisher's version; http://dx.doi.org/10.1111/bpa.12927Test; Sí; Brain Pathology 31(2): 365-380 (2021); http://hdl.handle.net/10261/262390Test; http://dx.doi.org/10.13039/501100014180Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100004587Test; http://dx.doi.org/10.13039/501100000780Test |
| DOI: | 10.1111/bpa.12927 |
| DOI: | 10.13039/501100014180 |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100004587 |
| DOI: | 10.13039/501100000780 |
| الإتاحة: | https://doi.org/10.1111/bpa.12927Test https://doi.org/10.13039/501100014180Test https://doi.org/10.13039/501100003329Test https://doi.org/10.13039/501100004587Test https://doi.org/10.13039/501100000780Test http://hdl.handle.net/10261/262390Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.687D25EE |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10156305 17503639 |
|---|---|
| DOI: | 10.1111/bpa.12927 |