التفاصيل البيبلوغرافية
العنوان: |
Data_Sheet_2_A Novel mcr-1 Variant Carried by an IncI2-Type Plasmid Identified From a Multidrug Resistant Enterotoxigenic Escherichia coli.ZIP |
المؤلفون: |
Hongbo Liu, Binghua Zhu, Beibei Liang, Xuebin Xu, Shaofu Qiu, Leili Jia, Peng Li, Lang Yang, Yongrui Li, Ying Xiang, Jing Xie, Ligui Wang, Chaojie Yang, Yansong Sun, Hongbin Song |
سنة النشر: |
2018 |
المجموعة: |
Frontiers: Figshare |
مصطلحات موضوعية: |
Microbiology, Microbial Genetics, Microbial Ecology, Mycology, mobilized colistin resistance (mcr-1) gene, novel mcr-1 variant, clinical diarrhea, enterotoxigenic Escherichia coli (ETEC), multidrug resistance (MDR) |
الوصف: |
In this study, we discovered a novel mobilized colistin resistance (mcr-1) gene variant, named mcr-1.9, which was identified in a colistin-resistant enterotoxigenic Escherichia coli (ETEC) strain from a clinical diarrhea case. The mcr-1.9 gene differs from mcr-1 at position 1036 due to a single nucleotide polymorphism (G→A), which results in an aspartic acid residue being replaced by an asparagine residue (Asp346→Asn) in the MCR-1 protein sequence. Antimicrobial susceptibility testing showed that the mcr-1.9-harboring ETEC strain is resistant to colistin at a minimum inhibitory concentration of 4 μg/ml. Plasmid profiling and conjugation experiments also suggest that the mcr-1.9 variant can be successfully transferred into the E. coli strain J53, indicating that the gene is located on a transferable plasmid. Bioinformatics analysis of data obtained from genome sequencing indicates that the mcr-1.9 gene is located on a 64,005 bp plasmid which has been named pEC26. This plasmid was found to have high similarity to the mcr-1-bearing IncI2-type plasmids pWF-5-19C (99% identity and 99% coverage) and pmcr1-IncI2 (99% identity and 98% coverage). The mcr-1.9-harboring ETEC also shows multidrug resistance to nine classes of antibiotics, and contains several virulence and antimicrobial-resistance genes suggested by the genome sequence analysis. Our report is the first to identify a new mcr-1 variant in an ETEC isolated from a human fecal sample, raising concerns about the existence of more such variants in human intestinal flora. Therefore, we believe that an undertaking to identify new mcr-1 variants in the bacterial communities of human intestines is of utmost importance, and that measures need to be taken to control the spread of mcr-1 and its variants in human intestinal microflora. |
نوع الوثيقة: |
dataset |
اللغة: |
unknown |
العلاقة: |
https://figshare.com/articles/dataset/Data_Sheet_2_A_Novel_mcr-1_Variant_Carried_by_an_IncI2-Type_Plasmid_Identified_From_a_Multidrug_Resistant_Enterotoxigenic_Escherichia_coli_ZIP/6180260Test |
DOI: |
10.3389/fmicb.2018.00815.s002 |
الإتاحة: |
https://doi.org/10.3389/fmicb.2018.00815.s002Test https://figshare.com/articles/dataset/Data_Sheet_2_A_Novel_mcr-1_Variant_Carried_by_an_IncI2-Type_Plasmid_Identified_From_a_Multidrug_Resistant_Enterotoxigenic_Escherichia_coli_ZIP/6180260Test |
حقوق: |
CC BY 4.0 |
رقم الانضمام: |
edsbas.67046AC1 |
قاعدة البيانات: |
BASE |